Val66Met 功能多态性与脑源性神经营养因子（BDNF）在精神分裂症和抑郁症急性发作中的血清蛋白水平。
Val66Met functional polymorphism and serum protein level of brain-derived neurotrophic factor (BDNF) in acute episode of schizophrenia and depression.
机构信息
Laboratory of Psychiatric Genetics, Department of Psychiatry, Poznan University of Medical Sciences, Poznań, Poland.
Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
出版信息
Pharmacol Rep. 2018 Feb;70(1):55-59. doi: 10.1016/j.pharep.2017.08.002. Epub 2017 Aug 12.
BACKGROUND
Brain-derived neurotrophic factor (BDNF) influences neuron differentiation during development as well as the synaptic plasticity and neuron survival in adulthood. BDNF has been implicated in the pathogenesis of schizophrenia and depression. Val66Met polymorphism and BDNF serum level are potential biomarkers in neuropsychiatric disorders. The aim of this study was to determine the effect of BDNF gene Val66Met functional polymorphism on serum BDNF concentration in patients with schizophrenia, during depression episode and in healthy control group.
METHODS
183 participants were recruited (61 patients with depressive episode, 56 females with schizophrenia, 66 healthy controls) from Polish population. Serum BDNF levels were measured using ELISA method. Val66Met polymorphism was genotyped using PCR- RFLP method.
RESULTS
Serum BDNF levels were not associated with Val66Met polymorphism in either of the groups. A significant increase of BDNF level in schizophrenia (p = 0.0005) and depression (p = 0.026) comparing to the control group has been observed.
CONCLUSIONS
Our results suggest that the functional Val66Met BDNF polymorphism is not associated with BDNF serum levels, which is in line with previous findings. Replication studies on larger groups are needed.
背景
脑源性神经营养因子（BDNF）在发育过程中影响神经元分化，以及成年后的突触可塑性和神经元存活。BDNF 与精神分裂症和抑郁症的发病机制有关。Val66Met 多态性和 BDNF 血清水平是神经精神疾病的潜在生物标志物。本研究旨在确定 BDNF 基因 Val66Met 功能多态性对精神分裂症患者、抑郁发作期间和健康对照组血清 BDNF 浓度的影响。
方法
从波兰人群中招募了 183 名参与者（61 名抑郁发作患者、56 名女性精神分裂症患者、66 名健康对照组）。使用 ELISA 法测量血清 BDNF 水平。使用 PCR-RFLP 方法对 Val66Met 多态性进行基因分型。
结果
在任何一组中，血清 BDNF 水平均与 Val66Met 多态性无关。与对照组相比，精神分裂症（p=0.0005）和抑郁症（p=0.026）患者的 BDNF 水平显著升高。
结论
我们的结果表明，功能性 Val66Met BDNF 多态性与 BDNF 血清水平无关，这与之前的研究结果一致。需要在更大的群体中进行复制研究。
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