Mandić Jelena Juri, Kozmar Ana, Kusačić-Kuna Sanja, Jazbec Anamarija, Mandić Krešimir, Mrazovac Danijela, Vukojević Nenad
Department of Ophthalmology, Clinical Hospital Centre Zagreb, Medical School University of Zagreb, Kispaticeva 12, 10000, Zagreb, Croatia.
Department of Laboratory Diagnostics, Clinical Hospital Centre Zagreb, Zagreb, Croatia.
Graefes Arch Clin Exp Ophthalmol. 2018 Apr;256(4):845-852. doi: 10.1007/s00417-017-3892-6. Epub 2018 Jan 14.
Simultaneous analyses of the contents and ratios of 12 cytokines and growth factors in single samples of human tears were performed, and the results were compared between a group of healthy subjects and a group of patients with Graves' hyperthyreosis (GH) without thyroid-associated orbitopathy (TAO).
Determinations and concentration measurements of interleukins (IL-2, IL4, IL-6, IL-8, IL-10, IL-1α, and IL-1β) interferon (IFN-γ), tumor necrosis factor (TNF-α), monocyte chemoattractant protein (MCP-1), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were performed with single tear samples from 21 patients with hyperthyreosis and 22 healthy subjects. The analyses were performed using a Randox microchip with an Evidence Biochip Array Analyzer.
We found significant differences between the healthy donor group and the hyperthyreosis group in the levels of IL-6, IL-10, VEGF, IL-1α, and MCP-1. The concentration of IL-6 was considerably higher in the hyperthyreosis group, IL-10 was higher in the healthy donor group, and VEGF and MPC-1 were higher in the hyperthyreosis group. The IL-8 and IFN-γ levels were higher in the hyperthyreosis group. The ratios of all of the cytokines to anti-inflammatory IL-10 were significantly elevated in the hyperthyreosis group.
There are clear differences in the levels of cytokines and growth factors in the tears of healthy subjects and patients with GH without TAO. Tear cytokine changes and related dysfunctional tear syndrome (DTS) could be an early sign of occult TAO in Graves' hyperthyreosis patients.
对人眼泪单一样本中的12种细胞因子和生长因子的含量及比例进行同步分析，并比较健康受试者组与一组无甲状腺相关眼病（TAO）的格雷夫斯甲状腺功能亢进症（GH）患者之间的结果。
对21例甲状腺功能亢进症患者和22例健康受试者的单份眼泪样本进行白细胞介素（IL-2、IL4、IL-6、IL-8、IL-10、IL-1α和IL-1β）、干扰素（IFN-γ）、肿瘤坏死因子（TNF-α）、单核细胞趋化蛋白（MCP-1）、血管内皮生长因子（VEGF）和表皮生长因子（EGF）的测定及浓度测量。使用带有Evidence生物芯片阵列分析仪的兰道克斯微芯片进行分析。
我们发现健康供体组和甲状腺功能亢进症组在IL-6、IL-10、VEGF、IL-1α和MCP-1水平上存在显著差异。甲状腺功能亢进症组中IL-6的浓度明显更高，健康供体组中IL-10更高，而甲状腺功能亢进症组中VEGF和MPC-1更高。甲状腺功能亢进症组中IL-8和IFN-γ水平更高。甲状腺功能亢进症组中所有细胞因子与抗炎性IL-10的比例均显著升高。
健康受试者和无TAO的GH患者眼泪中的细胞因子和生长因子水平存在明显差异。眼泪细胞因子变化及相关的功能性泪液综合征（DTS）可能是格雷夫斯甲状腺功能亢进症患者隐匿性TAO的早期迹象。
