Broda Martyna, Van Aken Olivier
ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, Australia.
Department of Biology, Lund University, Lund, Sweden.
Methods Mol Biol. 2018;1743:73-85. doi: 10.1007/978-1-4939-7668-3_7.
Cellular homeostasis requires precise communication between various types of organelles. In particular, the communication between nucleus and semiautonomous organelles, mitochondria and chloroplasts, has received widespread attention. Communication from nucleus to other organelles is known as anterograde signaling, whereas communication from mitochondria or chloroplasts to the nucleus is known as retrograde signaling. Here we discuss methods used to study retrograde signaling in Arabidopsis thaliana. These methods may also be modified to study retrograde signaling in other plant species.
细胞稳态需要各种细胞器之间进行精确的通讯。特别是，细胞核与半自主细胞器（线粒体和叶绿体）之间的通讯受到了广泛关注。从细胞核到其他细胞器的通讯被称为正向信号传导，而从线粒体或叶绿体到细胞核的通讯则被称为逆向信号传导。在这里，我们讨论了用于研究拟南芥中逆向信号传导的方法。这些方法也可以进行修改，以研究其他植物物种中的逆向信号传导。
