Doccula Fabrizio Gandolfo, Luoni Laura, Behera Smrutisanjita, Bonza Maria Cristina, Costa Alex
Department of Biosciences, University of Milan, Milan, Italy.
Methods Mol Biol. 2018;1743:125-141. doi: 10.1007/978-1-4939-7668-3_12.
Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).Here we describe an innovative and noninvasive protocol based on in vivo bioimaging technique coupled with utilization of genetically encoded fluorescent sensors that allows to monitor and analyze intracellular calcium (Ca) dynamics and changes of the glutathione redox status taking place in plant organs during plant interaction with the HR-inducing bacteria Pseudomonas syringae (PstAvrB).
植物通过启动适当且高效的下游防御反应来应对病原微生物的攻击，这些反应通常涉及一种称为过敏反应（HR）的局部细胞死亡形式。在此，我们描述了一种基于体内生物成像技术并结合使用基因编码荧光传感器的创新且非侵入性方案，该方案能够监测和分析植物器官在与诱导HR的丁香假单胞菌（PstAvrB）相互作用期间细胞内钙（Ca）动态变化以及谷胱甘肽氧化还原状态的变化。
