Ise W, Haiker H, Weiss H
EMBO J. 1985 Aug;4(8):2075-80. doi: 10.1002/j.1460-2075.1985.tb03894.x.
The rotenone sensitive NADH:ubiquinone was isolated from mitochondria of Neurospora crassa as a monodisperse preparation with the apparent mol. wt. in Triton solution of 0.9 X 10(6). The enzyme is composed of at least 22 subunits with apparent mol. wts. in SDS between 70 and 11 kd. Six of the subunits with the mol. wts. 70, 48, 37, 25, 22 and 18 kd were radioactively labelled in the enzyme isolated from cells which had incorporated [35S]methionine in the presence of cycloheximide. These subunits are synthesized in the mitochondria. Eleven subunits were radioactively labelled in the enzyme from cells which had incorporated [35S]methionine in the presence of chloramphenicol. These subunits are synthesized in the cytoplasm. The site of translation of the other subunits could not be established by the pulse-labelling technique. The assignment of the mitochondrially synthesized subunits to unidentified reading frames on the mitochondrial DNA is discussed.
从粗糙脉孢菌线粒体中分离出对鱼藤酮敏感的NADH：泛醌，作为一种单分散制剂，在Triton溶液中的表观分子量为0.9×10⁶。该酶由至少22个亚基组成，在SDS中的表观分子量在70至11kd之间。在从在环己酰亚胺存在下掺入[³⁵S]甲硫氨酸的细胞中分离出的酶中，分子量为70、48、37、25、22和18kd的六个亚基被放射性标记。这些亚基在线粒体中合成。在氯霉素存在下掺入[³⁵S]甲硫氨酸的细胞的酶中，有11个亚基被放射性标记。这些亚基在细胞质中合成。其他亚基的翻译位点无法通过脉冲标记技术确定。讨论了线粒体合成的亚基与线粒体DNA上未鉴定阅读框的对应关系。
