基于大豆过氧化物酶标记抗体作为信号放大物的电化学发光检测心肌肌钙蛋白 I。
Electrochemiluminescent detection of cardiac troponin I by using soybean peroxidase labeled-antibody as signal amplifier.
机构信息
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Nanjing Second Hospital, China.
出版信息
Talanta. 2018 Apr 1;180:47-53. doi: 10.1016/j.talanta.2017.12.015. Epub 2017 Dec 6.
This work proposed an electrochemiluminescence (ECL) immunosensor for quantitative monitoring of cardiac troponin I (cTnI) in plasma with soybean peroxidase (SBP) labeled-antibody as signal amplifier. The ECL sandwich immunosensor was constructed by covalent binding anti-cTnI capture antibody (Ab) to polyethylenimine-functionalized graphene matrix, which was obtained by a simple hydrothermal reaction between polyethylenimine (PEI) and graphene oxide (GO). After that, the SBP-labeled detection antibody (SBP-Ab), synthesized by NaIO method, was immobilized on the surface of electrode through sandwich immunoreaction. The SBP on electrode surface displayed strong and stable ECL signal of luminol in the presence of HO, which could be used for cTnI detection with a concentration range of 5-30,000pg/mL and a detection limit of 3.3pg/mL. This proposed SBP-modified immunosensor displayed high sensitivity, selectivity and accuracy, and was expected not only to detect cTnI in practical human plasma sample but also to be used in other biomarkers detection.
这项工作提出了一种基于电化学发光（ECL）的免疫传感器，用于定量监测血浆中心肌肌钙蛋白 I（cTnI），其信号放大采用大豆过氧化物酶（SBP）标记的抗体。ECL 三明治免疫传感器通过将抗 cTnI 捕获抗体（Ab）共价结合到聚乙烯亚胺功能化的石墨烯基质上来构建，该基质是通过聚乙烯亚胺（PEI）和氧化石墨烯（GO）之间的简单水热反应获得的。然后，通过夹心免疫反应将通过 NaIO 方法合成的 SBP 标记的检测抗体（SBP-Ab）固定在电极表面上。在存在 HO 的情况下，电极表面上的 SBP 显示出鲁米诺的强而稳定的 ECL 信号，可用于检测浓度范围为 5-30,000pg/mL、检测限为 3.3pg/mL 的 cTnI。该提出的 SBP 修饰的免疫传感器具有高灵敏度、选择性和准确性，不仅有望检测实际人血浆样品中的 cTnI，还可用于其他生物标志物的检测。
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