Kumar Mahesh, Govindrajan Jeyabalan, Nyola Narendra Kumar
Faculty of Pharmacy, Rajasthan University of Health Sciences, Jaipur, India.
Department of Pharmacognosy, Alwar College of Pharmacy, Alwar, Rajasthan, India.
Pharmacognosy Res. 2017 Dec;9(Suppl 1):S23-S26. doi: 10.4103/pr.pr_18_17.
Indian traditional system of medicine uses for the treatment of diabetes mellitus.
This study aims to develop an extract rich in saponins derived from seeds of the plant and to evaluate its antihyperglycemic potential and .
Defatted seeds were extracted with methanol and processed to afford saponin-enriched fraction ( saponin-enriched fraction [PDSEF]). This fraction was evaluated for its potential to inhibit enzymes such as α-glucosidase and α-amylase, . The fraction was subjected to oral toxicity study followed by sucrose tolerance test. An analytical high-performance liquid chromatography method was developed for fingerprinting of the fraction.
The method adopted for enrichment of saponins was robust enough to enrich saponin content to 96.37% ±1.21% w/w. PDSEF displayed superior inhibition of enzymes (α-glucosidase and α-amylase with IC of 5.12 ± 0.15 μg/ml and 17.28 ± 0.23 μg/ml, respectively) compared to acarbose. It was found to be safe in mice up to 2000 mg/kg and significantly prevented blood glucose level in sucrose tolerance test by inhibiting enzymes responsible for hydrolysis of sucrose.
PDSEF displayed excellent antihyperglycemic activity and and should be evaluated further to develop it as a promising drug for the management of diabetes mellitus.
Saponin enriched fraction from P. dulce seeds showed significant inhibition of key enzymes responsible for digestion of polysaccharides. The saponin enriched fraction was found to be safe in mice and prevented blood glucose level in mice in sucrose tolerance test. PDSEF: Pithecellobium dulce saponin-enriched fraction, IC50: Inhibitory concentration 50, HPLC: High performance liquid chromatography.
印度传统医学体系使用[具体内容缺失]来治疗糖尿病。
本研究旨在开发一种从该植物种子中提取的富含皂苷的提取物，并评估其降血糖潜力及[具体内容缺失]。
用甲醇提取脱脂种子并进行处理，以获得富含皂苷的部分（甜荚豆富含皂苷部分[PDSEF]）。评估该部分对α-葡萄糖苷酶和α-淀粉酶等酶的抑制潜力，[具体内容缺失]。对该部分进行口服毒性研究，随后进行蔗糖耐量试验。开发了一种分析型高效液相色谱法用于该部分的指纹图谱分析。
所采用的皂苷富集方法足够稳健，可将皂苷含量富集至96.37%±1.21%（w/w）。与阿卡波糖相比，PDSEF对酶（α-葡萄糖苷酶和α-淀粉酶，IC50分别为5.12±0.15μg/ml和17.28±0.23μg/ml）表现出更强的抑制作用。在小鼠中，高达2000mg/kg时发现其是安全的，并且在蔗糖耐量试验中通过抑制负责蔗糖水解的酶显著降低了血糖水平。
PDSEF表现出优异的降血糖活性及[具体内容缺失]，应进一步评估以将其开发成为一种有前景的糖尿病治疗药物。
甜荚豆种子的富含皂苷部分对负责多糖消化的关键酶表现出显著抑制作用。在小鼠中发现该富含皂苷部分是安全的，并且在蔗糖耐量试验中可降低小鼠的血糖水平。PDSEF：甜荚豆富含皂苷部分，IC50：半数抑制浓度，HPLC：高效液相色谱法
