Je Jae-Young, Park Soo Yeon, Ahn Chang-Bum
Department of Marine-Bio Convergence Science, Pukyong National University, Busan 48547, Korea.
Department of Marine Bio-Food Science, Chonnam National University, Jeonnam 59626, Korea.
Prev Nutr Food Sci. 2017 Dec;22(4):312-319. doi: 10.3746/pnf.2017.22.4.312. Epub 2017 Dec 31.
Rhizoid of was hydrolyzed with proteases and carbohydrases to obtain antioxidant materials. Oxygen radical absorbance capacity (ORAC) of the enzymatic extracts was evaluated and the Protamex extract (PE) exhibited the highest ORAC value. PE also potently scavenged 2,2-diphenyl-1-picrylhydrazyl radical, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic) acid cation radical, and hydrogen peroxide (HO) and had good reducing power. PE inhibited hydroxyl radical-induced DNA scission by measuring the conversion of supercoiled pBR322 plasmid DNA to the open circular form. The cytoprotective effect of PE against HO-induced hepatic cell damage was also investigated. PE showed a dose-dependent cytoprotective effect in cultured hepatocytes by inhibiting intracellular reactive oxygen species scavenging activity. In addition, PE up-regulated the expression of heme oxygenase-1, which is a cytoprotective enzyme, by activating translocation of nuclear factor-erythroid 2-related factor 2. Taken together, the enzymatic extract of rhizoid of , particularly PE, may be useful for antioxidant additives.
对[植物名称]的假根用蛋白酶和碳水化合物酶进行水解以获得抗氧化物质。对酶提取物的氧自由基吸收能力（ORAC）进行了评估，其中Protamex提取物（PE）表现出最高的ORAC值。PE还能有效清除2,2-二苯基-1-苦基肼自由基、2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）阳离子自由基和过氧化氢（HO），并具有良好的还原能力。通过测量超螺旋pBR322质粒DNA向开环形式的转化，PE抑制了羟基自由基诱导的DNA断裂。还研究了PE对HO诱导的肝细胞损伤的细胞保护作用。PE通过抑制细胞内活性氧清除活性，在培养的肝细胞中表现出剂量依赖性的细胞保护作用。此外，PE通过激活核因子-红细胞2相关因子2的易位，上调了具有细胞保护作用的血红素加氧酶-1的表达。综上所述，[植物名称]假根的酶提取物，尤其是PE，可能对抗氧化添加剂有用。
