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HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY.大肠杆菌突变体中半乳糖代谢的遗传性缺陷，II. 半乳糖诱导的敏感性
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Acceptance by Erwinia spp. of R plasmid R68.45 and its ability to mobilize the chromosome of Erwinia chrysanthemi.欧文氏菌属对R质粒R68.45的接受情况及其动员菊欧文氏菌染色体的能力。
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7
Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi.利用质粒pULB113（RP4::mini-Mu）构建胡萝卜软腐欧文氏菌菊花亚种的连锁图谱。
J Bacteriol. 1983 Jun;154(3):1489-92. doi: 10.1128/jb.154.3.1489-1492.1983.
8
Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.通过RP4::Mu将噬菌体Mu导入各种属的细菌以及属间基因转移。
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Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种中的转座子Tn5诱变
J Bacteriol. 1984 Mar;157(3):809-14. doi: 10.1128/jb.157.3.809-814.1984.

用IncP质粒R68::Mu对胡萝卜软腐欧文氏菌胡萝卜软腐亚种进行染色体定位

Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu.

作者信息

Forbes K J, Pérombelon M C

出版信息

J Bacteriol. 1985 Dec;164(3):1110-6. doi: 10.1128/jb.164.3.1110-1116.1985.

DOI:10.1128/jb.164.3.1110-1116.1985
PMID:2933392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC219304/
Abstract

Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca. 10(-5) chromosomal recombinants per donor, the presence of a Mu prophage on the chromosome of the donor increased the frequency of mobilization of markers adjacent to the prophage by up to 10-fold. Using this system it was possible to order 17 chromosomal mutations. The behavior of Mu in E. carotovora subsp. carotovora was also studied.

摘要

通过使用质粒R68::Mu c+将染色体转移到多个突变受体中，在胡萝卜软腐欧文氏菌胡萝卜软腐亚种SCRI193中建立了接合基因转移。观察到，虽然单独的质粒以约每供体10^(-5)个染色体重组体的频率随机转移标记，但供体染色体上Mu原噬菌体的存在使与原噬菌体相邻的标记转移频率提高了多达10倍。利用该系统可以对17个染色体突变进行排序。还研究了Mu在胡萝卜软腐欧文氏菌胡萝卜软腐亚种中的行为。