用IncP质粒R68::Mu对胡萝卜软腐欧文氏菌胡萝卜软腐亚种进行染色体定位
Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu.
作者信息
Forbes K J, Pérombelon M C
出版信息
J Bacteriol. 1985 Dec;164(3):1110-6. doi: 10.1128/jb.164.3.1110-1116.1985.
Abstract
Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca. 10(-5) chromosomal recombinants per donor, the presence of a Mu prophage on the chromosome of the donor increased the frequency of mobilization of markers adjacent to the prophage by up to 10-fold. Using this system it was possible to order 17 chromosomal mutations. The behavior of Mu in E. carotovora subsp. carotovora was also studied.
摘要
通过使用质粒R68::Mu c+将染色体转移到多个突变受体中，在胡萝卜软腐欧文氏菌胡萝卜软腐亚种SCRI193中建立了接合基因转移。观察到，虽然单独的质粒以约每供体10^(-5)个染色体重组体的频率随机转移标记，但供体染色体上Mu原噬菌体的存在使与原噬菌体相邻的标记转移频率提高了多达10倍。利用该系统可以对17个染色体突变进行排序。还研究了Mu在胡萝卜软腐欧文氏菌胡萝卜软腐亚种中的行为。
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