用噬菌体Mu d1（Apr lac cts62）对胡萝卜软腐欧文氏菌胡萝卜软腐亚种进行诱变：his-lac基因融合体的构建
Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.
作者信息
Jayaswal R K, Bressan R A, Handa A K
出版信息
J Bacteriol. 1984 May;158(2):764-6. doi: 10.1128/jb.158.2.764-766.1984.
Abstract
The bacteriophage Mu d1(Apr lac cts62 ) obtained from an Escherichia coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid phage was utilized as a vector for phage mutagenesis in Erwinia carotovora subsp. carotovora. Among ampicillin-resistant transductants. 1.4% were auxotrophs. The synthesis of beta-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains.
摘要
从携带缺陷型Mu d1噬菌体和Mu - P1杂交噬菌体的大肠杆菌双溶源菌中获得的噬菌体Mu d1(Apr lac cts62 )被用作胡萝卜软腐欧文氏菌胡萝卜软腐亚种噬菌体诱变的载体。在氨苄青霉素抗性转导子中，1.4%是营养缺陷型。在两种不同的组氨酸 - 乳糖融合菌株中，组氨酸饥饿时β - 半乳糖苷酶的合成被去阻遏。
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