利用质粒pULB113（RP4::mini-Mu）构建胡萝卜软腐欧文氏菌菊花亚种的连锁图谱。
Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi.
作者信息
Schoonejans E, Toussaint A
出版信息
J Bacteriol. 1983 Jun;154(3):1489-92. doi: 10.1128/jb.154.3.1489-1492.1983.
Abstract
We report experimental evidence that pULB113, an RP4::mini-Mu plasmid, mediates chromosome transfer in a strain of Erwinia carotovora subsp. chrysanthemi which does not accept the F episome. This allowed us to construct a genetic map of that strain by measuring the frequencies of cotransfer of different markers (thy, leu, pro, [his, trp], thyA, rpsL, ile).
摘要
我们报告了实验证据，即RP4::mini-Mu质粒pULB113在胡萝卜软腐欧文氏菌菊花亚种的一个菌株中介导染色体转移，该菌株不接受F附加体。这使我们能够通过测量不同标记（thy、leu、pro、[his、trp]、thyA、rpsL、ile）的共转移频率来构建该菌株的遗传图谱。
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