Université de Strasbourg, CNRS, ICS UPR 22 , F-67000 Strasbourg, France.
Institute of Atomic and Molecular Science, Shaanxi University of Science & Technology , CN-710021 Xi'an, Shaanxi, China.
Langmuir. 2018 Feb 6;34(5):1981-1991. doi: 10.1021/acs.langmuir.7b01928. Epub 2018 Jan 26.
In this paper, superparamagnetic iron oxide nanoparticles (SPIONs, around 6 nm) encapsulated in poly(methyl methacrylate) nanoparticles (PMMA NPs) with controlled sizes ranging from 100 to 200 nm have been successfully produced. The hybrid polymeric NPs were prepared following two different methods: (1) nanoprecipitation and (2) nanoemulsification-evaporation. These two methods were implemented in two different microprocesses based on the use of an impact jet micromixer and an elongational-flow microemulsifier. SPIONs-loaded PMMA NPs synthesized by the two methods presented completely different physicochemical properties. The polymeric NPs prepared with the micromixer-assisted nanoprecipitation method showed a heterogeneous dispersion of SPIONs inside the polymer matrix, an encapsulation efficiency close to 100 wt %, and an irregular shape. In contrast, the polymeric NPs prepared with the microfluidic-assisted nanoemulsification-evaporation method showed a homogeneous dispersion, an almost complete encapsulation, and a spherical shape. The properties of the polymeric NPs have been characterized by dynamic light scattering, thermogravimetric analysis, and transmission electron microscope. In vitro cytotoxicity assays were also performed on the nanohybrids and pure PMMA NPs.
本文成功制备了尺寸在 100 至 200nm 之间、具有可控尺寸的聚甲基丙烯酸甲酯（PMMA）纳米粒子（NPs）包裹的超顺磁氧化铁纳米粒子（SPIONs，约 6nm）。采用两种不同的方法：（1）纳米沉淀法和（2）纳米乳液蒸发法，制备了这种杂化聚合物 NPs。这两种方法在两种不同的微工艺中实施，基于使用冲击射流微混合器和拉伸流微乳化器。通过这两种方法制备的负载 SPIONs 的 PMMA NPs 表现出完全不同的物理化学性质。用微混合器辅助的纳米沉淀法制备的聚合物 NPs 显示出 SPIONs 在聚合物基质中的不均匀分散，接近 100wt%的封装效率和不规则形状。相比之下，用微流控辅助的纳米乳液蒸发法制备的聚合物 NPs 显示出均匀的分散、几乎完全的封装和球形。通过动态光散射、热重分析和透射电子显微镜对聚合物 NPs 的性能进行了表征。还对纳米杂化物和纯 PMMA NPs 进行了体外细胞毒性测定。
