Cho Young-Keol, Kim Jung-Eun, Foley Brian T
1 Department of Microbiology, University of Ulsan College of Medicine , Seoul, South Korea .
2 HIV Databases, Theoretical Biology and Biophysics Group, Los Alamos National Laboratory , Los Alamos, New Mexico .
AIDS Res Hum Retroviruses. 2018 Apr;34(4):347-353. doi: 10.1089/AID.2017.0238. Epub 2018 Feb 12.
There have been no studies related to groups at the highest risk for HIV-1 infection in Korea before 1993. In this study, for the first time, we report the distribution of HIV subtypes in overseas sailors (OSs) and prostitutes who worked in brothels near U.S. military bases in Korea. We retrospectively determined the sequences of nef in 131 patients using reverse transcription polymerase chain reaction (RT-PCR). These patients composed of 102 OSs, 14 OS spouses, and 15 prostitutes. Phylogenetic analysis was performed using 128 Korean OSs, OS spouses, and prostitutes. The distribution of non-B subtypes (n = 105) was as follows: 39, CRF02_AG; 15, CRF01_AE; 7, A1; 7, A2; 6, D; 2, CRF06_cpx; 3, C; 6, G; 11, untypable; and 1 each for CRF09_cpx, CRF12_BF, CRF50_A1D, A3, AFG, H, F1, F2, and A. Of the 116 OSs and OS spouses, 101 (87%), 11 (9%), and 4 (3%) subjects had non-B, Western B, and Korean subclade B (KSB) HIV-1s, respectively. Among the 15 prostitutes, 10 had Western B (67%), 4 non-B (27%), and 1 KSB (7%) HIV-1s. All 14 couples, each comprising of an OS and his spouse, had the same subtype. KSB (5%) was detected in OSs and prostitutes in 1990 and 1994, respectively. Of the 131 patients analyzed in this study, 105 (80%), 21 (16%), and 5 (4%) were infected with the non-B, Western B, and KSB subtypes of HIV, respectively. In future, these data may provide an important foundation for analysis of HIV-1 subtypes in Korea.
1993年以前，韩国尚未开展与HIV-1感染高危人群相关的研究。在本研究中，我们首次报告了在韩国美军基地附近妓院工作的海外水手（OSs）和妓女中HIV亚型的分布情况。我们采用逆转录聚合酶链反应（RT-PCR）对131例患者的nef序列进行了回顾性测定。这些患者包括102名OSs、14名OS配偶和15名妓女。使用128名韩国OSs、OS配偶和妓女进行了系统发育分析。非B亚型（n = 105）的分布如下：CRF02_AG 39例；CRF01_AE 15例；A1 7例；A2 7例；D 6例；CRF06_cpx 2例；C 3例；G 6例；无法分型11例；CRF09_cpx、CRF12_BF、CRF50_A1D、A3、AFG、H、F1、F2和A各1例。在116名OSs和OS配偶中，分别有101例（87%）、11例（9%）和4例（3%）感染了非B、西方B和韩国B亚型分支（KSB）HIV-1。在15名妓女中，10例感染西方B（67%），4例感染非B（27%），1例感染KSB（7%）。所有14对由一名OS及其配偶组成的夫妇均感染相同亚型。1990年和1994年分别在OSs和妓女中检测到KSB（5%）。在本研究分析的131例患者中，分别有105例（80%）、21例（16%）和5例（4%）感染了HIV的非B、西方B和KSB亚型。未来，这些数据可能为韩国HIV-1亚型分析提供重要依据。
