Caramori T, Calogero S, Albertini A M, Galizzi A
Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Università di Pavia, Italy.
J Gen Microbiol. 1993 Jan;139(1):31-7. doi: 10.1099/00221287-139-1-31.
Three additional alleles of the outB gene of Bacillus subtilis, whose activity is required for spore outgrowth, were identified. The nucleotide sequence of three mutant genes was determined. Analyses of dominance-recessivity showed that the wild-type allele is dominant over the mutant ones. When the outB gene was placed under the control of the inducible spac-1 promoter, the presence of IPTG was necessary to obtain normal growth. The results suggested that the outB gene is required for growth of B. subtilis. Expression of outB from the sporulation promoter spoIID negatively affected subsequent spore outgrowth, without altering vegetative growth and sporulation.
鉴定出枯草芽孢杆菌outB基因的另外三个等位基因，芽孢萌发需要其活性。测定了三个突变基因的核苷酸序列。显性-隐性分析表明，野生型等位基因对突变型等位基因呈显性。当outB基因置于可诱导的spac-1启动子控制下时，需要加入异丙基-β-D-硫代半乳糖苷（IPTG）才能实现正常生长。结果表明，outB基因是枯草芽孢杆菌生长所必需的。从芽孢形成启动子spoIID表达outB对随后的芽孢萌发有负面影响，但不改变营养生长和芽孢形成。
