[血管细胞间黏附分子-1在小儿毛细支气管炎中的表达及其意义]
[Expression of vascular intercellular adhesion molecule-1 and its significance in children with bronchiolitis].
作者信息
Zhang Xiao-Ming, Zhu Hai-Tao, Chang Ming
机构信息
Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2018 Jan;20(1):43-47. doi: 10.7499/j.issn.1008-8830.2018.01.009.
OBJECTIVE
To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) in serum and induced sputum supernatant in children with bronchiolitis, as well as its role in the pathogenesis of bronchiolitis in children.
METHODS
A total of 67 children with bronchiolitis who were diagnosed and hospitalized between July 2015 and January 2017 were enrolled. According to the diagnostic criteria, these children were divided into mild group with 22 children, moderate group with 24 children, and severe group with 21 children. A total of 20 children who underwent physical examination were enrolled as healthy control group. ELISA was used to measure the level of ICAM-1 in serum and induced sputum supernatant in the children with bronchiolitis in the acute stage and recovery stage and the children in the healthy control group.
RESULTS
Compared with the healthy control group, the mild, moderate, and severe bronchiolitis groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the mild group, the moderate and severe groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the moderate group, the severe group had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the children with bronchiolitis in the acute stage, the children in the recovery stage had a significant reduction in the level of ICAM-1 in serum and sputum (P<0.01). The correlation analysis showed that in the acute stage, the level of ICAM-1 in serum was positively correlated with that in sputum in children with bronchiolitis (r=0.875, P<0.001).
CONCLUSIONS
ICAM-1 is involved in the pathogenesis of bronchiolitis and is associated with disease severity.
目的
探讨细胞间黏附分子-1（ICAM-1）在毛细支气管炎患儿血清及诱导痰上清液中的表达情况及其在小儿毛细支气管炎发病机制中的作用。
方法
选取2015年7月至2017年1月期间诊断并住院的67例毛细支气管炎患儿。根据诊断标准，将这些患儿分为轻症组22例、中症组24例、重症组21例。选取20例体检儿童作为健康对照组。采用酶联免疫吸附测定（ELISA）法检测毛细支气管炎急性期和恢复期患儿以及健康对照组儿童血清及诱导痰上清液中ICAM-1水平。
结果
与健康对照组相比，轻症、中症和重症毛细支气管炎组血清及痰液中ICAM-1水平显著升高（P<0.01）。与轻症组相比，中症和重症组血清及痰液中ICAM-1水平显著升高（P<0.01）。与中症组相比，重症组血清及痰液中ICAM-1水平显著升高（P<0.01）。与毛细支气管炎急性期患儿相比，恢复期患儿血清及痰液中ICAM-1水平显著降低（P<0.01）。相关性分析显示，急性期毛细支气管炎患儿血清中ICAM-1水平与痰液中ICAM-1水平呈正相关（r=0.875，P<0.001）。
结论
ICAM-1参与毛细支气管炎的发病机制，且与疾病严重程度相关。
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