T-Mediated Nanosensor for Immunoassay Based on an Activatable MnO Nanoassembly.

Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T)-based nanosensor by using Mn released from the reduction of a MnO nanoassembly that can induce the change of T, and thus can greatly improve the sensitivity and overcome the "hook effect" of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO nanoassembly, the T signal of Mn released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.