College of Biosystems Engineering and Food Science, Zhejiang University, Zhejiang, 310058, China.
Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang, 310058, China.
Adv Healthc Mater. 2024 Jul;13(18):e2304484. doi: 10.1002/adhm.202304484. Epub 2024 Mar 31.
Argonaute (Ago) as a powerful enzyme has provided new insights into biosensing due to its programmability, high sensitivity, and user-friendly operation. However, current strategies mainly rely on phosphorylated guide DNA to modulate the cleavage activity of Ago, which is limited in versatility and simplicity. Herein, the authors report the Mn-enhanced cleavage activity of Ago and employ Mn-ions with variable valence to regulate the activity of Pyrococcus furiosus Ago (PfAgo) for biosensing applications. The conversion of Mn ions with different valence states through MnO nanoflowers enables the sensitive detection of ascorbic acid, alkaline phosphatase, and arsenic with limits of detection of 2.5 nmol L, 0.009 U L, and 0.4 ng mL, respectively. A PfAgo-based immunoassay is further developed that allows for the detection of diverse targets, thus providing a promising toolbox to broaden PfAgo-based sensors into versatile bioanalytical and biomedical applications.
Argonaute（AGO）作为一种强大的酶，由于其可编程性、高灵敏度和用户友好的操作，为生物传感提供了新的见解。然而，目前的策略主要依赖于磷酸化的引导 DNA 来调节 AGO 的切割活性，这在多功能性和简单性方面受到限制。在此，作者报道了 AGO 的 Mn 增强的切割活性，并利用具有可变价态的 Mn 离子来调节 Pyrococcus furiosus AGO（PfAGO）的活性，用于生物传感应用。通过 MnO 纳米花实现 Mn 离子不同价态的转化，实现了对抗坏血酸、碱性磷酸酶和砷的灵敏检测，检测限分别为 2.5 nmol L、0.009 U L 和 0.4 ng mL。进一步开发了基于 PfAGO 的免疫分析，允许检测多种靶标，从而为将基于 PfAGO 的传感器扩展到多功能生物分析和生物医学应用提供了有前途的工具包。
