School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Nanomedicine (Lond). 2018 Feb;13(4):391-405. doi: 10.2217/nnm-2017-0297. Epub 2018 Jan 17.
To explore the hemostatic effect of Phellodendri Cortex-derived carbon dots.
MATERIALS & METHODS: Transmission electron microscopy, high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, fluorescence spectroscopy, x-ray photoelectron spectroscopy and a cell counting kit-8 assay were studied. Hemostatic effect of Phellodendri Cortex Carbonisatus-carbon dots (PCC-CDs) was studied in mouse bleeding models. To explore their related hemostatic mechanism, coagulation parameters and platelets were measured.
The PCC-CDs ranged in diameter from 1.2 to 4.8 nm and had a quantum yield of 9.62%. They exhibited no toxicity up to concentrations of 1000 μg/ml. After administration, mice had a significantly shortened bleeding time and coagulation parameters and platelets significantly increased.
These results showed the definite hemostatic effect of PCC-CDs.
探究黄柏炭衍生碳点的止血效果。
采用透射电子显微镜、高分辨率透射电子显微镜、傅里叶变换红外光谱、紫外-可见吸收光谱、荧光光谱、X 射线光电子能谱和细胞计数试剂盒-8 检测法进行研究。在小鼠出血模型中研究黄柏炭衍生碳点（PCC-CDs）的止血效果。为了探究其相关止血机制，测量了凝血参数和血小板。
PCC-CDs 的直径为 1.2 至 4.8nm，量子产率为 9.62%。其浓度高达 1000μg/ml 时也没有毒性。给药后，小鼠的出血时间明显缩短，凝血参数和血小板明显增加。
这些结果表明 PCC-CDs 具有明确的止血效果。
