Yan Xin, Zhao Yan, Luo Juan, Xiong Wei, Liu Xiaoman, Cheng Jinjun, Wang Yongzhi, Zhang Meiling, Qu Huihua
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
J Nanobiotechnology. 2017 Sep 5;15(1):60. doi: 10.1186/s12951-017-0296-z.
Pollen Typhae Carbonisata (PTC) is a type of calcined herb drug that has been used as a hemostatic medicine to promote hemostasis for thousands of years. In this study, we discovered and separated novel water-soluble carbon quantum dots (CQDs, named PTC-CQDs) from aqueous extracts of PTC. These PTC-CDs were characterized using transmission electron microscopy (TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet-visible, and fluorescence spectroscopy. Then, we assessed the anti-hemorrhagic effects and related hemostatic mechanisms of the obtained PTC-CQDs.
The PTC-CQDs separated from PTC are spherical, monodisperse, and have a narrow size distribution between 2 and 8 nm. In the pharmacology experiment, remarkable anti-hemorrhage effects of PTC-CQDs were revealed. Additionally, the rats showed a profound decrease in activated partial thromboplastin time and increase in fibrinogen and PLT after PTC-CQDs treatment.
These results indicated the explicit hemostasis effect of PTC-CQDs, which not only provided a new idea for the material research of PTC, but have also provided new insights into potential biomedical and healthcare applications of CQDs in the field of haemorrhage control and laid a solid foundation for future drug discovery.
蒲黄炭是一种煅制的草药，数千年来一直被用作止血药物以促进止血。在本研究中，我们从蒲黄炭的水提取物中发现并分离出新型水溶性碳量子点（CQDs，命名为PTC-CQDs）。使用透射电子显微镜（TEM）、高分辨率TEM以及傅里叶变换红外光谱、紫外可见光谱和荧光光谱对这些PTC-CQDs进行了表征。然后，我们评估了所获得的PTC-CQDs的抗出血作用及相关止血机制。
从蒲黄炭中分离出的PTC-CQDs呈球形，单分散，粒径在2至8nm之间分布狭窄。在药理学实验中，显示出PTC-CQDs具有显著的抗出血作用。此外，PTC-CQDs治疗后大鼠的活化部分凝血活酶时间显著缩短，纤维蛋白原和血小板计数增加。
这些结果表明PTC-CQDs具有明确的止血作用，这不仅为蒲黄炭的物质研究提供了新思路，也为碳量子点在出血控制领域潜在的生物医学和医疗保健应用提供了新见解，并为未来的药物发现奠定了坚实基础。
