基于二元共混纤维的循环肿瘤细胞捕获检测用于结直肠癌的临床诊断。
Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer.
机构信息
Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, 110, Taiwan.
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec. 4, Keelung Road, Taipei, 106, Taiwan, ROC.
出版信息
J Nanobiotechnology. 2018 Jan 16;16(1):4. doi: 10.1186/s12951-017-0330-1.
BACKGROUND
In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis.
METHODS
We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption.
RESULTS
Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs.
CONCLUSIONS
Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.
背景
除了传统方法外，在患者血液中检测和分析CTC 还可以早期诊断癌症转移。
方法
我们通过静电纺丝将聚环氧乙烷（PEO）混入尼龙-6 中，以生成基于纤维垫的循环肿瘤细胞（CTC）检测方法。优化电纺共混纤维垫（EBFM）中尼龙-6 和 PEO 的含量，以促进高细胞-基底亲和力和低白细胞吸附。
结果
与 IsoFlux 系统（一种用于 CTC 检测的商业仪器）相比，EBFM 的 CTC 检测显示出较低的假阳性读数，并且对临床前标本具有较高的灵敏度和选择性。此外，我们使用 CTC 检测来检查结直肠癌的临床诊断准确性，并将结果与结肠镜活检的病理分析进行比较。我们通过 CTC 检测对结直肠癌的阳性表达与通过对结肠镜活检的病理分析识别的完全吻合，适用于 II 期、III 期和 IV 期结直肠癌患者。然而，在通过结肠镜活检的病理分析识别为 I 期结直肠癌的四个人中，有两个人显示出 CTC 的阳性表达。有十个患者通过病理分析被识别为没有结直肠肿瘤。然而，这十个人中有两个人表现出 CTC 的阳性表达。
结论
因此，在该人群中，这种低成本的 EBFMs 表现出了相当高的对非侵入性结直肠癌诊断的捕获效率。
Zotero 插件