Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Institute for Biology, Freie Universität Berlin, 14195, Berlin, Germany.
Sci Rep. 2018 Jan 16;8(1):815. doi: 10.1038/s41598-017-18989-1.
Jumonji (JmjC) domain proteins are known regulators of gene expression and chromatin organization by way of histone demethylation. Chromatin modification and remodeling provides a means to modulate the activity of large numbers of genes, but the importance of this class of predicted histone-modifying enzymes for different aspects of post-developmental processes remains poorly understood. Here we test the function of all 11 non-lethal members in the regulation of circadian rhythms and sleep. We find loss of every Drosophila JmjC gene affects different aspects of circadian behavior and sleep in a specific manner. Together these findings suggest that the majority of JmjC proteins function as regulators of behavior, rather than controlling essential developmental programs.
Jumonji (JmjC) 结构域蛋白是已知的通过组蛋白去甲基化来调节基因表达和染色质组织的调控因子。染色质修饰和重塑为调节大量基因的活性提供了一种手段，但这类预测的组蛋白修饰酶在发育后过程的不同方面的重要性仍知之甚少。在这里，我们测试了调节生物钟和睡眠的 11 个非致死性成员的功能。我们发现，果蝇中每个 JmjC 基因的缺失都会以特定的方式影响生物钟行为和睡眠的不同方面。这些发现表明，大多数 JmjC 蛋白作为行为的调节剂发挥作用，而不是控制基本的发育程序。
