Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425, Jülich, Germany.
Appl Microbiol Biotechnol. 2018 Feb;102(4):1575-1585. doi: 10.1007/s00253-018-8747-5. Epub 2018 Jan 16.
Plants synthesize several thousand different polyphenols of which many have the potential to aid in preventing or treating cancer, cardiovascular, and neurodegenerative diseases. However, plants usually contain complex polyphenol mixtures impeding access to individual compounds in larger quantities. In contrast, functional integration of biosynthetic plant polyphenol pathways into microorganisms allows for the production of individual polyphenols as chemically distinct compounds, which can be synthesized in large amounts and can be more easily isolated. Over the last decade, microbial synthesis of many plant polyphenols could be achieved, and along the way, many decisive bottlenecks in the endogenous microbial host metabolism as well as in the heterologous plant pathways could be identified. In this review, we present recent advancements in metabolic engineering of microorganisms for the production of plant polyphenols and discuss how current challenges could be addressed in the future.
植物合成了几千种不同的多酚，其中许多具有预防或治疗癌症、心血管和神经退行性疾病的潜力。然而，植物通常含有复杂的多酚混合物，这阻碍了大量获取单个化合物的机会。相比之下，将生物合成植物多酚途径的功能整合到微生物中，可以生产出化学性质不同的单个多酚化合物，这些化合物可以大量合成，并且更容易分离。在过去的十年中，已经实现了许多植物多酚的微生物合成，在此过程中，可以确定内源性微生物宿主代谢以及异源植物途径中的许多决定性瓶颈。在这篇综述中，我们介绍了微生物生产植物多酚的代谢工程的最新进展，并讨论了未来如何应对当前的挑战。
