Marienhagen Jan
Institute of Bio- and Geosciences, Forschungszentrum Jülich, IBG-1: Biotechnology, 52425, Jülich, Germany.
Institute of Biotechnology, RWTH Aachen University, Worringer Weg 3, 52074, Aachen, Germany.
Appl Microbiol Biotechnol. 2025 May 30;109(1):132. doi: 10.1007/s00253-025-13520-3.
A significant proportion of industrially important small molecules are aromatic, and the majority of these compounds are produced chemically, relying heavily on fossil resources. In bacteria and plants, the shikimate pathway and related biosynthetic routes serve as the primary sources of aromatic compounds. Microbial cell factories, which are poised to play a central role in the emerging bio-based economy, provide a sustainable alternative for producing commercially valuable aromatics from renewable resources. Corynebacterium glutamicum, already established as an industrial workhorse for the large-scale production of various amino acids, can be engineered to overproduce aromatic compounds derived from the shikimate pathway. Furthermore, the functional integration of heterologous or synthetic pathways enables access to high-value natural products, such as plant polyphenols and other polyketides. This review highlights recent advancements in the metabolic engineering of C. glutamicum for the sustainable production of these aromatic compounds. KEY POINTS: • C. glutamicum's high tolerance to aromatic compounds is key to aromatics production. • Detailed physiological insights enable access to shikimate pathway-derived products. • Diverse plant (poly)phenols and other aromatic polyketides can be produced.
相当一部分具有工业重要性的小分子是芳香族的，并且这些化合物中的大多数是通过化学方法生产的，严重依赖化石资源。在细菌和植物中，莽草酸途径及相关生物合成途径是芳香族化合物的主要来源。微生物细胞工厂有望在新兴的生物基经济中发挥核心作用，为从可再生资源生产具有商业价值的芳香族化合物提供了一种可持续的替代方案。谷氨酸棒杆菌已成为大规模生产各种氨基酸的工业主力菌株，可以通过基因工程改造使其过量生产源自莽草酸途径的芳香族化合物。此外，异源或合成途径的功能整合能够获得高价值的天然产物，如植物多酚和其他聚酮化合物。本综述重点介绍了谷氨酸棒杆菌代谢工程在可持续生产这些芳香族化合物方面的最新进展。要点：•谷氨酸棒杆菌对芳香族化合物的高耐受性是生产芳香族化合物的关键。•详细的生理学见解有助于获得源自莽草酸途径的产物。•可以生产多种植物（多）酚和其他芳香族聚酮化合物。
