Zhang Jiangyang, Wu Dan, Turnbull Daniel H
Department of Radiology, Bernard and Irene Schwartz Center for Biomedical Imaging, New York University (NYU) School of Medicine, New York, NY, USA.
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2018;1718:285-296. doi: 10.1007/978-1-4939-7531-0_17.
Genetically engineered mouse models are used extensively as models of human development and developmental diseases. Conventional histological approaches are static and two-dimensional, and do not provide a full understanding of the dynamic, spatiotemporal changes in developing mouse embryos. Magnetic resonance imaging (MRI) offers a noninvasive and longitudinal approach for three-dimensional in utero imaging of normal and mutant mouse embryos. In this chapter, we describe MRI approaches that have been developed for imaging the living embryonic mouse brain and vasculature. Details are provided on the animal preparation and setup, MRI equipment, acquisition and reconstruction methods that have been found to be most useful for in utero MRI, including examples of applications to fetal mouse neuroimaging.
基因工程小鼠模型被广泛用作人类发育和发育性疾病的模型。传统的组织学方法是静态的二维方法，无法全面了解发育中小鼠胚胎的动态时空变化。磁共振成像（MRI）为正常和突变小鼠胚胎的子宫内三维成像提供了一种非侵入性的纵向方法。在本章中，我们描述了已开发出的用于对活体胚胎小鼠大脑和脉管系统进行成像的MRI方法。详细介绍了动物准备和设置、MRI设备、采集和重建方法，这些方法已被证明对子宫内MRI最为有用，包括胎儿小鼠神经成像的应用实例。
