Liu Jing-Min, Wang Zhi-Hao, Ma Hui, Wang Shuo
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University , 94 Weijin Road, Tianjin 300071, People's Republic of China.
J Agric Food Chem. 2018 Feb 7;66(5):1061-1066. doi: 10.1021/acs.jafc.7b05225. Epub 2018 Jan 29.
Development of real-time and in situ analytical methods for determination of food-borne pathogens and toxins ingested into the human body would be a promising research direction in the food-safety area. The present perspective starts with summarization of the up-to-date progress of the nanomaterial-assisted in vitro detection methods for pathogens and toxins and finally focuses on application of animal bioimaging to in vivo study, including prospective strategies for in vivo quantification of target pathogens or toxins and in vivo investigation of their behaviors inside the living body, with the assistance of real-time and non-invasive optical bioimaging. This perspective provides the advisory direction for food-safety research, from in vitro to in vivo, along with a prospective discussion of the further development roadmap of the food-safety detection techniques, especially the bioimaging-guided methods for investigation and mediation of the food contamination effect to human health.
开发用于测定摄入人体的食源性病原体和毒素的实时原位分析方法将是食品安全领域一个很有前景的研究方向。本综述首先总结了纳米材料辅助的病原体和毒素体外检测方法的最新进展，最后聚焦于动物生物成像在体内研究中的应用，包括在实时和非侵入性光学生物成像的辅助下，对目标病原体或毒素进行体内定量以及对其在生物体内行为进行体内研究的前瞻性策略。本综述为从体外到体内的食品安全研究提供了指导方向，同时对食品安全检测技术的进一步发展路线图进行了前瞻性讨论，特别是生物成像引导的用于研究和调节食品污染对人类健康影响的方法。
