宫颈鳞状细胞癌中与临床肿瘤分期相关的mRNA和miRNA的鉴定。

Identification of clinical tumor stages related mRNAs and miRNAs in cervical squamous cell carcinoma.

作者信息

Yang Chenggang, Ren Jing, Li Bangling, Zhang Dongmei, Ma Cui, Cheng Cheng, Sun Yaolan, Fu Lina, Shi Xiaofeng

机构信息

Gu'an Bojian Bio-Technology Co., LTD., Langfang, China; Department of BigData, Beijing Medintell Bioinformatic Technology Co., LTD., Beijing, China.

Department of BigData, Beijing Medintell Bioinformatic Technology Co., LTD., Beijing, China.

出版信息

Pathol Res Pract. 2018 Oct;214(10):1638-1647. doi: 10.1016/j.prp.2018.07.035. Epub 2018 Aug 3.

DOI:10.1016/j.prp.2018.07.035

Abstract

OBJECTIVES

The aim of this study is to identify the clinical tumor stage related mRNAs and miRNAs, shedding light on the potential molecular mechanisms of cervical squamous cell carcinoma (CSCC).

METHODS

Firstly, the mRNA and miRNA next-generation sequencing data were downloaded. Secondly, clinical tumor stage correlation analysis of mRNAs and miRNA was performed, followed by the functional enrichment analysis of all clinical tumor stage related mRNAs. Thirdly, differentially expression analysis of mRNAs and miRNA between different clinical tumor stages was performed, followed by target gene prediction of these differentially expressed miRNAs.

RESULTS

3 mRNAs (PER1, PRKAB1 and PMM2) and 5 miRNAs (hsa-mir-486, hsa-mir-451, hsa-mir-424, hsa-mir-144 and hsa-mir-450a-2) were overlapped from stage 1, stage 2, stage 3 and stage 4.

CONCLUSIONS

Alterations of differentially expressed mRNAs and miRNAs may offer important insights into the molecular mechanisms in the pathology of CSCC.

摘要

目的

本研究旨在鉴定与临床肿瘤分期相关的mRNA和miRNA，以阐明宫颈鳞状细胞癌（CSCC）的潜在分子机制。

方法

首先，下载mRNA和miRNA的下一代测序数据。其次，对mRNA和miRNA进行临床肿瘤分期相关性分析，随后对所有与临床肿瘤分期相关的mRNA进行功能富集分析。第三，对不同临床肿瘤分期之间的mRNA和miRNA进行差异表达分析，随后对这些差异表达的miRNA进行靶基因预测。

结果

在1期、2期、3期和4期中，有3种mRNA（PER1、PRKAB1和PMM2）和5种miRNA（hsa-mir-486、hsa-mir-451、hsa-mir-424、hsa-mir-144和hsa-mir-450a-2）存在重叠。

结论

差异表达的mRNA和miRNA的改变可能为CSCC病理学中的分子机制提供重要见解。