Kalra Prerna, Karwasra Ritu, Gupta Yogendra Kumar, Ray Subrata Basu, Singh Surender
a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
b Department of Anatomy , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
Nat Prod Res. 2019 Jun;33(11):1641-1645. doi: 10.1080/14786419.2018.1425843. Epub 2018 Jan 17.
In the present study, we have evaluated the nephroprotective effect of hydroalcoholic extract of in cisplatin-induced nephrotoxicity model. Standardised was orally administered to Wistar rats for 10 days at different doses. On day 7, 8 mg/kg of cisplatin was administered intra-peritoneally to rats in all groups. , in a dose-dependent manner significantly inhibited the elevation of serum creatinine, blood urea nitrogen and oxidant stress markers. The immunohistochemical analysis revealed the increased levels of apoptotic markers and cytokines in cisplatin group were significantly lowered by extract. The cisplatin-treated rats kidney showed diffused tubular necrosis and infilteration of inflammatory cells which was reversed in the treatment group. Chemical characterisation of extract by HPLC revealed the presence of corilagin, chebulinic, chebulagic, chebulic, gallic and ellagic acid. The findings of this study discovered that ameliorated oxidative and histological damage caused by cisplatin.
在本研究中，我们评估了[提取物名称]水醇提取物在顺铂诱导的肾毒性模型中的肾保护作用。将标准化的[提取物名称]以不同剂量口服给予Wistar大鼠，持续10天。在第7天，向所有组的大鼠腹腔注射8mg/kg顺铂。[提取物名称]以剂量依赖性方式显著抑制血清肌酐、血尿素氮和氧化应激标志物的升高。免疫组织化学分析显示，顺铂组凋亡标志物和细胞因子水平升高，而[提取物名称]提取物可显著降低这些水平。顺铂处理的大鼠肾脏显示弥漫性肾小管坏死和炎性细胞浸润，而在治疗组中这种情况得到逆转。通过高效液相色谱法对提取物进行化学表征，结果显示存在柯里拉京、诃子次酸、诃子酸、诃子鞣酸、没食子酸和鞣花酸。本研究结果发现，[提取物名称]改善了顺铂引起的氧化损伤和组织学损伤。
