Maly I P, Sasse D
Histochemistry. 1985;83(5):431-6. doi: 10.1007/BF00509205.
Microquantitative measurements of ADH-activity were carried out on the livers of male and female rats, guinea-pigs and horses (two geldings and a mare). Lyophilized cryostat sections of liver parenchyma were microdissected the whole way along the sinusoidal length from the terminal afferent vessels to the terminal efferent venule. ADH activity in samples of about 50-150 ng was measured in a microbiochemical assay using the oil-well technique without enzymatic cycling, by direct luminometric determination of NADH. On the basis of the single measurements, mean values of total hepatic ADH activity could be calculated and the specific distribution patterns graphically demonstrated. Total activity of ADH in the liver of the female rat is 1.6 times higher than in the male; the male distribution pattern exhibits a relative maximum in the intermediary zone of the acinus while the activity in the liver of female rats increases towards a perivenous maximum. Mean values for total ADH activity in the livers of male and female guinea-pigs are almost equal and there is, moreover, no clear intra-acinar gradient. Mare and castrated male horses show high hepatic ADH activity which is evenly distributed in the liver acinus.
对雄性和雌性大鼠、豚鼠以及马（两匹阉割公马和一匹母马）的肝脏进行了抗利尿激素（ADH）活性的微量定量测量。沿着肝实质的冷冻切片从终末传入血管到终末传出小静脉，沿着整个窦状隙长度进行显微解剖。使用油井技术，通过直接荧光法测定NADH，在无酶循环的微生物化学分析中测量约50 - 150纳克样品中的ADH活性。基于单次测量，可以计算出肝脏总ADH活性的平均值，并以图形方式展示特定的分布模式。雌性大鼠肝脏中ADH的总活性比雄性高1.6倍；雄性的分布模式在腺泡的中间区域呈现相对最大值，而雌性大鼠肝脏中的活性朝着静脉周围最大值增加。雄性和雌性豚鼠肝脏中ADH总活性的平均值几乎相等，而且腺泡内没有明显的梯度。母马和阉割公马的肝脏显示出高ADH活性，且在肝腺泡中均匀分布。
