Maly I P, Sasse D
Anatomisches Institut, Universität Basel, Switzerland.
Histochemistry. 1988;88(3-6):387-93. doi: 10.1007/BF00570299.
Total and low-Km aldehyde dehydrogenase (ALDH) activity was measured in 50-150 ng microdissected liver tissue samples of the entire sinusoidal length. High-Km ALDH activity was calculated by subtracting the low-Km ALDH values from the total ALDH activity. Enzyme activity was measured by a microchemical assay, using the oil-well technique with luminometric determination of NADH. The intra-acinar profiles of high-Km and low-Km ALDH activity could be demonstrated graphically for both male and female rats after 84 h of starvation, and after starvation and refeeding for 6 nights. In addition, the ALDH distribution patterns of juvenile, castrated, and castrated and testosterone-treated rats were determined. It could be demonstrated that starvation, and starvation followed by refeeding, lead to changes in enzyme activity which parallel the loss and regain of liver- and body-weight. The nutritional factors do not essentially alter the normal intra-acinar profiles. In juvenile rats, ALDH is lower by 30% in comparison with the controls, but sex-differences in the distribution profiles are not yet present. Castration has no effect on the amount of enzyme activity but the sex specific distribution profiles are less marked. The main effect of testosterone treatment is an elevation of low-Km ALDH in the perivenous zone. The characteristics of the intra-acinar profiles of high-Km and low-Km ALDH activity are discussed with respect to hepatic acetaldehyde oxidation and alcoholic liver damage.
在整个肝血窦长度上对50 - 150纳克显微切割的肝组织样本进行总醛脱氢酶（ALDH）和低Km醛脱氢酶活性的测定。高Km ALDH活性通过从总ALDH活性中减去低Km ALDH值来计算。采用油井技术结合NADH的化学发光测定法，通过微量化学分析来测量酶活性。对于饥饿84小时后以及饥饿并再喂养6个晚上后的雄性和雌性大鼠，均可通过图形展示高Km和低Km ALDH活性的腺泡内分布情况。此外，还测定了幼年、去势以及去势并经睾酮处理的大鼠的ALDH分布模式。结果表明，饥饿以及饥饿后再喂养会导致酶活性发生变化，这与肝脏和体重的减轻及恢复情况平行。营养因素并未从根本上改变正常的腺泡内分布情况。在幼年大鼠中，与对照组相比，ALDH降低了30%，但分布模式尚无性别差异。去势对酶活性量没有影响，但性别特异性分布模式不太明显。睾酮处理的主要作用是使肝静脉周围区域的低Km ALDH升高。结合肝脏乙醛氧化和酒精性肝损伤，对高Km和低Km ALDH活性的腺泡内分布特征进行了讨论。
