Zhao Jing, Li Bo, Shu Chuqiang, Ma Yun, Gong Yingping
Department of Gynecology, Hunan Provincial Maternal and Child Health Hospital, Changsha, Hunan 410008, P.R. China.
Oncol Lett. 2017 Dec;14(6):7437-7442. doi: 10.3892/ol.2017.7114. Epub 2017 Oct 2.
Accumulating studies have revealed that microRNAs serve crucial roles in cancer development and progression. MicroRNA-30a (miR-30a) has been implicated in various cancer types. However, the role of miR-30a in cervical cancer remains unclear. In the current study, a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay revealed that miR-30a was significantly downregulated in cervical cancer tissues compared with adjacent normal tissues, and in the cervical cancer cell lines HeLa, SiHa and Ca-Ski compared with GH329 normal cervical epithelial cells. A functional assay using miR-30a mimic demonstrated that miR-30a could inhibit the growth and invasion of cervical cancer cells. Additionally, bioinformatics-based prediction and luciferase reporter assays indicated that is a direct target of miR-30a. Transfection with miR-30a reduced the mRNA expression and protein levels of , as determined using RT-qPCR and western blot analyses. Furthermore, expression was negatively correlated with that of miR-30a in cervical cancers. Overall, the present study demonstrated that miR-30a functions as a tumor suppressor by targeting in cervical cancer, which may provide the basis for a prognostic biomarker or therapeutic strategy for cervical cancer.
越来越多的研究表明，微小RNA在癌症的发生和发展中起着至关重要的作用。微小RNA-30a（miR-30a）已被证明与多种癌症类型有关。然而，miR-30a在宫颈癌中的作用仍不清楚。在本研究中，逆转录-定量聚合酶链反应（RT-qPCR）分析显示，与相邻正常组织相比，miR-30a在宫颈癌组织中显著下调，与GH329正常宫颈上皮细胞相比，在宫颈癌细胞系HeLa、SiHa和Ca-Ski中也显著下调。使用miR-30a模拟物的功能分析表明，miR-30a可以抑制宫颈癌细胞的生长和侵袭。此外，基于生物信息学的预测和荧光素酶报告基因分析表明，[此处原文缺失具体基因名称]是miR-30a的直接靶点。用miR-30a转染可降低[此处原文缺失具体基因名称]的mRNA表达和蛋白水平，这是通过RT-qPCR和蛋白质印迹分析确定的。此外，在宫颈癌中，[此处原文缺失具体基因名称]的表达与miR-30a的表达呈负相关。总体而言，本研究表明，miR-30a在宫颈癌中通过靶向[此处原文缺失具体基因名称]发挥肿瘤抑制作用，这可能为宫颈癌的预后生物标志物或治疗策略提供依据。
