Luan Nianxu, Wang Yi, Liu Xuedong
Department of Pneumology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.
Oncol Lett. 2018 Jul;16(1):1173-1179. doi: 10.3892/ol.2018.8719. Epub 2018 May 16.
The microRNA (miR)-30 family has been reported to be aberrantly expressed in several types of cancer. However, its contributions to lung cancer remain to be fully elucidated. Myocyte enhancer factor 2D (MEF2D), an oncogene in liver cancer, has been shown to be aberrantly expressed in lung cancer. In the present study, it was found that MEF2D and miR-30a were inversely correlated in lung cancer samples. Using an online database, it was predicted that miR-30a targeted the 3' untranslated region (UTR) of MEF2D mRNA. The activity of luciferase with MEF2D 3'UTR was suppressed by transfecting cells with miR-30a mimics. The results of western blot analysis showed that the miR-30a mimics also suppressed the MEF2D protein. The miR-30a mimics were able to reduce the growth and colonies of lung cancer cells by suppressing MEF2D. The results of FACS and western blot assays showed that the apoptotic rate was reduced by transfection with the miR-30a mimics. Collectively, the aberrant expression of miR-30a in lung cancer promoted the expression of MEF2D protein. miR-30a inhibited the growth and colony formation of the lung cancer cells by promoting apoptosis.
据报道，微小RNA（miR）-30家族在多种癌症类型中表达异常。然而，其对肺癌的作用仍有待充分阐明。肌细胞增强因子2D（MEF2D）是一种肝癌中的癌基因，已被证明在肺癌中表达异常。在本研究中，发现肺癌样本中MEF2D与miR-30a呈负相关。使用在线数据库预测，miR-30a靶向MEF2D mRNA的3'非翻译区（UTR）。用miR-30a模拟物转染细胞可抑制带有MEF2D 3'UTR的荧光素酶活性。蛋白质印迹分析结果表明，miR-30a模拟物也可抑制MEF2D蛋白。miR-30a模拟物能够通过抑制MEF2D来减少肺癌细胞的生长和集落形成。流式细胞术和蛋白质印迹分析结果表明，转染miR-30a模拟物可降低凋亡率。总体而言，肺癌中miR-30a的异常表达促进了MEF2D蛋白的表达。miR-30a通过促进凋亡抑制肺癌细胞的生长和集落形成。
