Menzi Nadia, Osinga Rik, Todorov Atanas, Schaefer Dirk Johannes, Martin Ivan, Scherberich Arnaud
Department of Biomedicine, University Hospital Basel, University of Basel, Hebelstrasse 20, 4031, Basel, Switzerland.
Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital Basel, Spitalstrasse 21, 4031, Basel, Switzerland.
Cytotechnology. 2018 Apr;70(2):807-817. doi: 10.1007/s10616-018-0190-z. Epub 2018 Jan 17.
The isolation of stromal vascular fraction (SVF) cells from excised human adipose tissue, for clinical or research purposes, implies the tedious and time consuming process of manual mincing prior to enzymatic digestion. Since no efficient alternative technique to this current standard procedure has been proposed so far, the aim of this study was to test a milling procedure, using two simple, inexpensive and commercially available manual meat grinders, to process large amounts of adipose tissue. The procedure was assessed on adipose tissue resections from seven human donors and compared to manual mincing with scalpels. The processed adipose tissues were digested and the resulting SVF cells compared in terms of number, clonogenicity and differentiation capacity. After 10 min of processing, either device tested yielded on average sixfold more processed material for subsequent cell isolation than manual mincing. The isolation yield of SVF cells (isolated cells per ml of adipose tissue), their viability, phenotype, clonogenicity and osteogenic/adipogenic differentiation capacity, tested by production of mineralized matrix and lipid vacuoles, respectively, were comparable. This new method is practical and inexpensive and represents an efficient alternative to the current standard for large scale adipose tissue resection processing. A device based on the milling principle could be embedded within a streamlined system for isolation and clinical use of SVF cells from adipose tissue excision.
从切除的人体脂肪组织中分离基质血管成分（SVF）细胞，用于临床或研究目的，这意味着在酶消化之前需要进行繁琐且耗时的手动切碎过程。由于目前尚未提出比现行标准程序更有效的替代技术，本研究的目的是测试一种研磨程序，使用两种简单、廉价且市售的手动绞肉机来处理大量脂肪组织。该程序在来自七名人类捐赠者的脂肪组织切除样本上进行评估，并与使用手术刀进行手动切碎的方法进行比较。对处理后的脂肪组织进行消化，并比较所得SVF细胞在数量、克隆形成能力和分化能力方面的差异。经过10分钟的处理，所测试的任何一种设备平均产生的用于后续细胞分离的处理材料都比手动切碎多六倍。通过分别检测矿化基质和脂质空泡的产生来测试的SVF细胞的分离产量（每毫升脂肪组织中分离出的细胞数）、活力、表型、克隆形成能力以及成骨/成脂分化能力均具有可比性。这种新方法实用且成本低廉，是大规模脂肪组织切除处理现行标准的一种有效替代方法。基于研磨原理的设备可以嵌入到一个简化系统中，用于从脂肪组织切除中分离SVF细胞并用于临床。
