Lin M T, Chern Y F, Chen S Y
Neuropharmacology. 1985 Nov;24(11):1039-42. doi: 10.1016/0028-3908(85)90188-1.
The effects of pretreatment of rats with an intrahypothalamic injection of 6-hydroxydopamine on the thermal responses induced by intrahypothalamic injection of noradrenaline, prostaglandin E2, thyrotropin-releasing hormone or beta-endorphin were assessed. Administration of either noradrenaline (2-10 micrograms), prostaglandin E2 (10-40 ng), thyrotropin-releasing hormone (0.5-2.0 micrograms) or beta-endorphin (1-3 micrograms) into the preoptic anterior hypothalamus caused a dose-dependent rise in rectal temperature in conscious rats at an ambient temperature of 22 degrees C. In addition, it was found that three intrahypothalamic doses of 10 micrograms of 6-hydroxydopamine at intervals of 2 days caused a significant depletion of noradrenaline in the hypothalamus to 26.4% of control while the concentration of dopamine in the hypothalamus was not significantly reduced at 95.3% of control. Furthermore, the hyperthermic responses induced by prostaglandin E2, thyrotropin-releasing hormone, or beta-endorphin were greatly attenuated after selective depletion of noradrenaline in the hypothalamus in rats. However, selective depletion of noradrenaline did not affect the noradrenaline-induced hyperthermic responses. The data indicate that either prostaglandin E2, thyrotropin-releasing hormone or beta-endorphin may act through the endogenous release of noradrenaline from the hypothalamus to induce hyperthermic responses in rats.
评估了对大鼠进行下丘脑内注射6-羟基多巴胺预处理后，下丘脑内注射去甲肾上腺素、前列腺素E2、促甲状腺激素释放激素或β-内啡肽所诱导的热反应。在22摄氏度的环境温度下，向清醒大鼠的视前区下丘脑前部注射去甲肾上腺素（2 - 10微克）、前列腺素E2（10 - 40纳克）、促甲状腺激素释放激素（0.5 - 2.0微克）或β-内啡肽（1 - 3微克），会导致直肠温度呈剂量依赖性升高。此外，还发现每隔2天进行3次下丘脑内注射10微克的6-羟基多巴胺，会使下丘脑内的去甲肾上腺素显著耗竭至对照水平的26.4%，而下丘脑内多巴胺的浓度未显著降低，为对照水平的95.3%。此外，在大鼠下丘脑内去甲肾上腺素被选择性耗竭后，前列腺素E2、促甲状腺激素释放激素或β-内啡肽所诱导的体温升高反应会大大减弱。然而，去甲肾上腺素的选择性耗竭并不影响去甲肾上腺素所诱导的体温升高反应。数据表明，前列腺素E2、促甲状腺激素释放激素或β-内啡肽可能通过下丘脑内源性释放去甲肾上腺素来诱导大鼠的体温升高反应。
