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独角莲不同溶剂提取物的植物化学筛选及抗菌活性

Phytochemical screening and antibacterial activity of different solvent extracted samples of Arisaema jacquemontii.

作者信息

Iqbal Madiha, Bakht Jehan, Shafi Mohammad

机构信息

Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, KPK, Pakistan.

Department of Agronomy, The University of Agriculture, Peshawar, KPK, Pakistan.

出版信息

Pak J Pharm Sci. 2018 Jan;31(1):75-81.

Abstract

The current research was carried out to assess the antibacterial activities and phytochemical analysis of the methanol, n-hexane, ethyl acetate, n-butanol soluble fractions and aqueous extracts of the tubers of Arisaema jacquemontii. All the extracts were tested for their antibacterial potential at 1, 2 and 3 mg disc concentrations against 6 bacterial strains through disc diffusion suseptibility assay. The data suggested that different extracts showed varying degree of growth inhibition against the tested microbes. Statistical analysis revealed that n-hexane and ethyl acetate soluble fractions significantly inhibited the growth of all the bacterial strains at the tested concentrations. Moderate activities were recorded for n-butanol and methanolic extracted samples at different concentrations against all the tested strains of bacteria. P. aeruginosa, S. aureus and X. campestris showed resistance to all the tested concentrations of the aqueous extract. B. subtilis and K. pneumoniae were resistant at 1 and 2 mg disc-1 concentrations of the aqueous extract and 3 mg disc-1 of the same extract reduced the growth of the same bacteria. Phytochemical analysis of the different solvent extracted samples suggested the presence or absence of various metabolites including alkaloids, saponins, tannins, sterols, flavonoids, protein, carbohydrates and fats.

摘要

开展当前研究以评估天南星块茎的甲醇、正己烷、乙酸乙酯、正丁醇可溶级分及水提取物的抗菌活性和植物化学分析。通过纸片扩散药敏试验,在1、2和3毫克纸片浓度下针对6种细菌菌株测试了所有提取物的抗菌潜力。数据表明,不同提取物对受试微生物表现出不同程度的生长抑制。统计分析显示,正己烷和乙酸乙酯可溶级分在测试浓度下显著抑制了所有细菌菌株的生长。不同浓度的正丁醇和甲醇提取物样品对所有受试细菌菌株表现出中等活性。铜绿假单胞菌、金黄色葡萄球菌和野油菜黄单胞菌对水提取物的所有测试浓度均表现出抗性。枯草芽孢杆菌和肺炎克雷伯菌在水提取物1和2毫克/纸片浓度下表现出抗性,而相同提取物3毫克/纸片浓度则抑制了相同细菌的生长。对不同溶剂提取物样品的植物化学分析表明存在或不存在包括生物碱、皂苷、单宁、甾醇、黄酮类、蛋白质、碳水化合物和脂肪在内的各种代谢物。

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