Khan Shehla, Bakht Jehan, Shafi Mohammad
Institute of Biotechnology and Genetic Engineering, The University of Agriculture. Peshawar. KPK. Pakistan.
Department of Agronomy, The University of Agriculture. Peshawar. KPK. Pakistan.
Pak J Pharm Sci. 2018 Jul;31(4(Supplementary)):1457-1461.
The present study investigates the antinociceptive, antimicrobial activity and phytochemical assessment of samples from A. pentapomicum. Different microbes were tested using disc diffusion assay at three concentrations (1, 2 and 3 mg/disc). Antinociceptive activity was determined by acetic acid induced and hot plate methods. The tested plant extracts revealed significant antinociceptive activity at dose dependent manner when measured by acetic acid induced method. The growth of all the tested microbes was inhibited by methanol, butanol, and ethyl acetate extracted samples at all concentrations. Chloroform, n-hexane and aqueous extracts inhibited the growth of the different microbes at high concentration. The most susceptible microbe was P. aeruginosa measuring 94% and 79% zone of inhibition by butanol and ethyl acetate extracts at 3mg /disc. C. fruendii, however, was the most resistant bacterium followed by S. aureus. The presence of alkaloids, proteins, amino acids, carbohydrates, flavonoids, tannins, saponins, and fats were confirmed in phytochemical screening of different extracts.
本研究调查了五棱苹果样品的抗伤害感受、抗菌活性及植物化学评估。使用纸片扩散法在三种浓度（1、2和3毫克/纸片）下对不同微生物进行测试。通过醋酸诱导法和热板法测定抗伤害感受活性。当采用醋酸诱导法测量时，受试植物提取物呈现出剂量依赖性的显著抗伤害感受活性。所有受试微生物的生长均受到甲醇、丁醇和乙酸乙酯提取物在所有浓度下的抑制。氯仿、正己烷和水提取物在高浓度下抑制不同微生物的生长。最敏感的微生物是铜绿假单胞菌，丁醇和乙酸乙酯提取物在3毫克/纸片时的抑菌圈分别为94%和79%。然而，弗氏柠檬酸杆菌是最具抗性的细菌，其次是金黄色葡萄球菌。在不同提取物的植物化学筛选中证实了生物碱、蛋白质、氨基酸、碳水化合物、黄酮类、单宁、皂苷和脂肪的存在。
