Zuskova E, Piackova V, Machova J, Chupani L, Steinbach C, Stara A, Velisek J
Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.
J Fish Dis. 2018 Apr;41(4):643-649. doi: 10.1111/jfd.12764. Epub 2018 Jan 19.
This study evaluated efficacy and toxicity of the pyrazinoisoquinoline anthelmintic praziquantel (PZQ) in barbel infected with metacercariae of Diplostomum spathaceum and adult Pomphorhynchus laevis, and assessed antioxidant biomarkers and the lipid peroxidation response in juvenile barbel post-treatment. The estimated 96-hr LC50 of PZQ was 28.6 mg/L. For evaluation of efficacy, barbel naturally infected with D. spathaceum were exposed to a 10 and 20 mg/L PZQ 4-day bath treatment. Both concentrations were 100% effective against D. spathaceum and significantly (p < .01) affected the activity of catalase, superoxide dismutase, glutathione reductase and glutathione-S-transferase as well as levels of reduced glutathione in liver and muscle. The efficacy of orally administered PZQ was assessed in adult barbel naturally infected with P. laevis. Fish were administered 10, 30 and 50 mg/kg of body weight and examined via gut dissection after 6 days. The 50 mg/kg dose significantly decreased the intensity of infection. Praziquantel is a feasible bath treatment for barbel infected with D. spathaceum and has potential for oral treatment of broodfish infected with P. laevis.
本研究评估了吡嗪异喹啉驱虫药吡喹酮（PZQ）对感染了匙形双腔吸虫尾蚴和成年平滑吸口鲶的鲃鱼的疗效和毒性，并评估了处理后幼鲃的抗氧化生物标志物和脂质过氧化反应。PZQ的估计96小时半数致死浓度（LC50）为28.6毫克/升。为评估疗效，将自然感染匙形双腔吸虫的鲃鱼置于10毫克/升和20毫克/升的PZQ中进行为期4天的浸浴处理。两种浓度对匙形双腔吸虫均100%有效，且显著（p < 0.01）影响了过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶的活性以及肝脏和肌肉中还原型谷胱甘肽的水平。对自然感染平滑吸口鲶的成年鲃鱼评估了口服PZQ的疗效。给鱼投喂10、30和50毫克/千克体重的药物，并在6天后通过解剖肠道进行检查。50毫克/千克的剂量显著降低了感染强度。吡喹酮是治疗感染匙形双腔吸虫鲃鱼的一种可行的浸浴处理方法，并且有口服治疗感染平滑吸口鲶亲鱼的潜力。
