Baldini L, Cortelezzi A, Polli N, Neri A, Nobili L, Maiolo A T, Lambertenghi-Deliliers G, Polli E E
Blood. 1986 Feb;67(2):458-64.
Isolated splenic hairy cells from three untreated patients were cultured in presence of recombinant human interferon alpha-2C (IFN alpha). Ultrastructural cytochemistry and immunophenotype analysis with a large panel of monoclonal antibodies were performed to study cellular modification induced by IFN alpha. Hairy cells showed a typical pheno-type: Smlg+, B1+, BA1+/-, anti-Tac+, OKDR+, Leu-M5+, HC2+, TRAP+, myeloperoxidase-. Under our experimental conditions, we found no direct cytotoxic effects or significant variations in morphology, cytochemistry, and percentage of reactivity with the tested monoclonal antibodies. After culturing in the presence of different doses of IFN alpha, we observed a significant enhancement of the expression of class II HLA antigens as demonstrated by increased fluorescence for OKDR, OKla, Leu-10 at fluorescence-activated cell sorting analysis. In agreement with this finding IFN alpha-treated hairy cells showed an increased stimulatory capacity v allogeneic T cells in one-way mixed lymphocyte reaction. To our knowledge this is the first report describing the induction of class II HLA antigens on hairy cells by IFN alpha.
从三名未经治疗的患者中分离出的脾毛细胞，在重组人干扰素α-2C（IFNα）存在的情况下进行培养。使用大量单克隆抗体进行超微结构细胞化学和免疫表型分析，以研究IFNα诱导的细胞变化。毛细胞表现出典型的表型：Smlg +、B1 +、BA1 + / -、抗Tac +、OKDR +、Leu-M5 +、HC2 +、TRAP +、髓过氧化物酶 -。在我们的实验条件下，我们未发现直接的细胞毒性作用，也未发现形态学、细胞化学以及与测试单克隆抗体反应百分比的显著变化。在不同剂量的IFNα存在下培养后，通过荧光激活细胞分选分析中OKDR、OKla、Leu-10荧光增加表明，我们观察到II类HLA抗原表达显著增强。与这一发现一致，在单向混合淋巴细胞反应中，经IFNα处理的毛细胞对同种异体T细胞的刺激能力增强。据我们所知，这是第一份描述IFNα诱导毛细胞上II类HLA抗原的报告。
