LMI CNRS UMR 5615, Université Lyon 1, Villeurbanne, 69622, France.
LIMMS/CNRS-IIS UMI 2820, Institute of Industrial Science, The University of Tokyo, Tokyo, 153-8505, Japan.
Sci Rep. 2018 Jan 19;8(1):1226. doi: 10.1038/s41598-018-19712-4.
The couple Calix[4]arene-1,3-O-diphosphorous acid (C4diP) and zinc ions (Zn) acts as a synergistic DNA binder. Silicon NanoTweezer (SNT) measurements show an increase in the mechanical stiffness of DNA bundles by a factor of >150, at Zn to C4diP ratios above 8, as compared to Zinc alone whereas C4diP alone decreases the stiffness of DNA. Electroanalytical measurements using 3D printed devices demonstrate a progression of events in the assembly of C4diP on DNA promoted by zinc ions. A mechanism at the molecular level can be deduced in which C4diP initially coordinates to DNA by phosphate-phosphate hydrogen bonds or in the presence of Zn by Zn bridging coordination of the phosphate groups. Then, at high ratios of Zn to C4diP, interdigitated dimerization of C4diP is followed by cross coordination of DNA strands through Zn/C4diP inter-strand interaction. The sum of these interactions leads to strong stiffening of the DNA bundles and increased inter-strand binding.
钙黄绿素-1,3-O-二膦酸（C4diP）和锌离子（Zn）作为协同 DNA 结合物。硅纳米镊子（SNT）测量表明，与单独的锌相比，当 Zn 与 C4diP 的比例高于 8 时，DNA 束的机械硬度增加了>150 倍，而单独的 C4diP 会降低 DNA 的硬度。使用 3D 打印设备的电化分析测量表明，在锌离子促进下，C4diP 在 DNA 上的组装过程中会发生一系列事件。可以推断出在分子水平上的一种机制，其中 C4diP 最初通过磷酸-磷酸氢键与 DNA 配位，或者在 Zn 的存在下通过磷酸基团的 Zn 桥配位进行配位。然后，在 Zn 与 C4diP 的高比例下，C4diP 交错二聚化，然后通过 Zn/C4diP 链间相互作用交联 DNA 链。这些相互作用的总和导致 DNA 束的强烈硬化和增加的链间结合。
