Arany Petra, Róka Eszter, Mollet Laurent, Coleman Anthony W, Perret Florent, Kim Beomjoon, Kovács Renátó, Kazsoki Adrienn, Zelkó Romána, Gesztelyi Rudolf, Ujhelyi Zoltán, Fehér Pálma, Váradi Judit, Fenyvesi Ferenc, Vecsernyés Miklós, Bácskay Ildikó
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary.
ICBMS, UMR 5246, Université Lyon 1, F69622 Villeurbanne, France.
Pharmaceutics. 2019 Jun 13;11(6):277. doi: 10.3390/pharmaceutics11060277.
3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The polyvalent test plates (PVTPs) are poly-lactic acid cylinders, 14 mm in diameter and 3 mm in height. The polymer ester backbone was surface modified by a series of ramified and linear oligoamines to increase its hydrophilicity and introduce a positive charge. The chemical modification was verified by FT-IR spectroscopy, showing the introduction of amide and amine functions, and contact angle measurements confirmed increased hydrophilicity. Morphology studies (SEM, optical microscopy) indicated that the modification of PVTP possessed a planar morphology with small pits. Positron annihilation lifetime spectroscopy demonstrated that the polymeric free volume decreased on modification. An MTT-based prolonged cytotoxicity test using Caco-2 cells showed that the PVTPs are non-toxic at the cellular level. The presence of surface oligoamines on the PVTPs reduced biofilm formation by SC5314 significantly. The results demonstrate that 3D printed objects may be modified at their surface by a simple amidation reaction, resulting in a reduced propensity for biofilm colonization and cellular toxicity.
3D打印因其能够快速生产原型和进行小批量生产而备受关注。熔融沉积成型（FDM）被用于制造多价测试板，以研究打印材料的物理、化学和体外生物学特性。多价测试板（PVTPs）是直径为14毫米、高度为3毫米的聚乳酸圆柱体。聚合物酯主链通过一系列分支状和线性低聚胺进行表面改性，以增加其亲水性并引入正电荷。通过傅里叶变换红外光谱（FT-IR）对化学改性进行了验证，结果表明引入了酰胺和胺官能团，接触角测量证实亲水性增加。形态学研究（扫描电子显微镜、光学显微镜）表明，PVTP的改性具有带有小坑的平面形态。正电子湮没寿命谱表明，改性后聚合物的自由体积减小。使用Caco-2细胞进行的基于MTT的长期细胞毒性测试表明，PVTP在细胞水平上无毒。PVTP表面的低聚胺显著减少了SC5314生物膜的形成。结果表明，3D打印物体可以通过简单的酰胺化反应在其表面进行改性，从而降低生物膜定植和细胞毒性的倾向。
