Irving W L, Walker P R, Lydyard P M
Ann Rheum Dis. 1985 Jul;44(7):462-8. doi: 10.1136/ard.44.7.462.
Blood lymphocytes from 53 patients with rheumatoid arthritis (RA) and 44 controls were cultured with the polyclonal B cell activator Epstein-Barr virus (EBV). Culture supernatants were removed at weekly intervals and the amount of IgM secreted by the lymphocytes measured by an enzyme-linked immunosorbent assay (ELISA). Three major differences in the pattern of EBV-induced IgM synthesis by RA versus control lymphocytes were observed. Lymphocytes from RA patients, in general, produced less IgM after one week in culture than controls. In contrast, they increased their IgM secretion significantly by the end of the second week, whereas control lymphocyte cultures showed little change in IgM secretion at this time. Control lymphocytes from EBV seropositive individuals produced undetectable amounts of IgM after five weeks in culture. However, lymphocytes from 40% of the RA patients, even though they were EBV seropositive, secreted greater than 2000 ng/ml (microgram/l) IgM after five weeks. The data are discussed in terms of defective B and T cell responses to EBV in lymphocytes from patients with RA.
用多克隆B细胞激活剂爱泼斯坦-巴尔病毒（EBV）培养53例类风湿性关节炎（RA）患者和44例对照者的血液淋巴细胞。每周去除培养上清液，并用酶联免疫吸附测定（ELISA）法测定淋巴细胞分泌的IgM量。观察到RA患者与对照淋巴细胞在EBV诱导的IgM合成模式上有三个主要差异。一般来说，RA患者的淋巴细胞在培养一周后产生的IgM比对照者少。相反，到第二周结束时，它们的IgM分泌显著增加，而此时对照淋巴细胞培养物的IgM分泌几乎没有变化。来自EBV血清阳性个体的对照淋巴细胞在培养五周后产生的IgM量检测不到。然而，40%的RA患者的淋巴细胞，即使他们是EBV血清阳性，在五周后分泌的IgM大于2000 ng/ml（微克/升）。从RA患者淋巴细胞中B细胞和T细胞对EBV反应缺陷的角度对数据进行了讨论。
