Susulovska Solomia, Castillo Pablo, Archidona-Yuste Antonio
Ivan Franko National University of Lviv, 79005 Lviv, Ukraine.
Institute for Sustainable Agriculture (IAS), CSIC, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Spain.
J Nematol. 2017 Dec;49(4):396-402.
Seven needle nematode species of the genus have been reported in Ukraine. Nematological surveys for needle nematodes were carried out in Ukraine between 2016 and 2017 and two nematode species of ( and ) were collected from natural and anthropogenically altered habitats on the territory of Opillia and Zakarpattia in Ukraine. Nematodes were extracted from 500 cm of soil by modified sieving and decanting method. Extracted specimens were processed to glycerol and mounted on permanent slides and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted as previously described for D2-D3 expansion segments of 28S rRNA. Sequence alignments for D2-D3 from showed 97%-99% similarity to other sequences of deposited in GenBank from Belgium, Bulgaria, Czech Republic, Russia, Slovenia, and Scotland. Similarly, D2-D3 sequence alignments from , showed 99% to other sequences of deposited in GenBank from Slovakia and Czech Republic. Morphology, morphometry, and molecular data obtained from these samples were consistent with and identification. To our knowledge, these are the first reports of and in Ukraine, extending the geographical distribution of these species.
乌克兰已报道过该属的七种针状线虫。2016年至2017年期间，在乌克兰开展了针对针状线虫的线虫学调查，从乌克兰奥皮利亚和外喀尔巴阡地区的自然和人为改变的栖息地中采集到了该属的两种线虫（和）。采用改良过筛和倾析法从500立方厘米的土壤中提取线虫。将提取的标本用甘油处理并固定在永久载玻片上，随后进行形态学和分子鉴定。从单个个体中提取线虫DNA，并按照先前描述的针对28S rRNA的D2-D3扩增片段的方法进行PCR分析。来自的D2-D3序列比对显示，与比利时、保加利亚、捷克共和国、俄罗斯、斯洛文尼亚和苏格兰存入GenBank的其他的序列有97%-99%的相似性。同样，来自的D2-D3序列比对显示，与斯洛伐克和捷克共和国存入GenBank的其他的序列有99%的相似性。从这些样本中获得的形态学、形态测量学和分子数据与和的鉴定结果一致。据我们所知，这些是和在乌克兰的首次报道，扩展了这些物种的地理分布范围。
