Clavero-Camacho Ilenia, Cantalapiedra-Navarrete Carolina, Kormpi Maria, Palomares-Rius Juan E, Tzortzakakis Emmanuel A, Castillo Pablo, Archidona-Yuste Antonio
Institute for Sustainable Agriculture (IAS), CSIC, Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004, Córdoba, Spain.
Benaki Phytopathological Institute, Kifisia, Athens, Greece.
J Nematol. 2022 Jul 29;54(1):20220027. doi: 10.2478/jofnem-2022-0027. eCollection 2022 Feb.
Longidorid nematodes comprise more than 500 species, and and are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, northern Greece, and one nematode species of () was recovered. Nematodes were extracted from soil samples by modified sieving and a decanting method. Extracted specimens were processed using glycerol, mounted on permanent slides, and subsequently identified morphologically. Nematode DNA was extracted from individual, live specimens, and PCR (Polymerase Chain Reaction) assays were performed for D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial regions. Morphology and morphometric data obtained from this population were consistent with the original description and reported populations of . To our knowledge, this is the first report of in Greece and the second in the Mediterranean Basin after the record of the species from Slovenia, extending the geographical distribution of this species in Europe.
长针线虫目线虫包含500多个物种，并且是植物寄生线虫中最多样化、分布最广泛且遍布全球的类属。该属包含一组物种，其中一些是植物病毒的传播媒介。在希腊北部塞萨洛尼基的一个葡萄种植区进行了针对针属线虫的新采样，并发现了一种针属（）线虫物种。通过改良的筛分和倾析法从土壤样本中提取线虫。提取的标本用甘油处理，固定在永久载玻片上，随后进行形态学鉴定。从单个活标本中提取线虫DNA，并对28S rRNA的D2 - D3扩增片段、ITS1 rRNA和部分线粒体区域进行聚合酶链反应（PCR）检测。从该群体获得的形态学和形态测量数据与该物种的原始描述以及已报道群体一致。据我们所知，这是该物种在希腊的首次报道，也是继在斯洛文尼亚有记录之后在地中海盆地的第二次报道，扩展了该物种在欧洲的地理分布范围。
