Ferreiro María José, Pérez Coralia, Marchesano Mariana, Ruiz Santiago, Caputi Angel, Aguilera Pedro, Barrio Rosa, Cantera Rafael
Departamento de Biología del Neurodesarrollo, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Center of Cooperative Research in Biosciences CIC bioGUNE, Bizkaia Technology Park, Derio, Spain.
Front Neurosci. 2018 Jan 4;11:732. doi: 10.3389/fnins.2017.00732. eCollection 2017.
Key scientific discoveries have resulted from genetic studies of , using a multitude of transgenic fly strains, the majority of which are constructed in a genetic background containing mutations in the gene. Here we report that mutant flies from strain undergo retinal degeneration. We observed also that mutants have progressive loss of climbing ability, shortened life span, as well as impaired resistance to various forms of stress. Retinal degeneration was abolished by transgenic expression of in the null background . We conclude that beyond the classical eye-color phenotype, mutations in gene could impair several biological functions affecting parameters like mobility, life span and stress tolerance. Consequently, we suggest caution and attentiveness during the interpretation of old experiments employing mutant flies and when planning new ones, especially within the research field of neurodegeneration and neuroprotection. We also encourage that the use of strain as a wild-type control should be avoided.
关键的科学发现来自于对[具体对象]的基因研究，使用了大量的转基因果蝇品系，其中大多数是在一个包含[基因名称]基因突变的遗传背景中构建的。在这里我们报告，来自[品系名称]品系的[基因名称]突变果蝇会发生视网膜退化。我们还观察到，[基因名称]突变体具有攀爬能力的逐渐丧失、寿命缩短以及对各种形式应激的抗性受损。在[基因名称]缺失背景[具体背景名称]中通过转基因表达[基因名称]可消除视网膜退化。我们得出结论，除了经典的眼色表型外，[基因名称]基因的突变可能会损害几种生物学功能，影响诸如运动能力、寿命和应激耐受性等参数。因此，我们建议在解释使用[基因名称]突变果蝇的旧实验以及规划新实验时要谨慎和留意，特别是在神经退行性变和神经保护研究领域。我们还鼓励避免将[品系名称]品系用作野生型对照。
