Rosas Mariel, Cantera Rafael, Prieto Daniel
Departamento de Biología del Neurodesarrollo, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Departamento de Neurofisiología Celular y Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
MicroPubl Biol. 2024 Nov 1;2024. doi: 10.17912/micropub.biology.001336. eCollection 2024.
We investigated the role of oxygen-sensing atypical guanylyl cyclase subunit Gyc89Db in the developing brain. Despite its expression in the hypoxic neuroepithelium of the larval optic lobe of , loss-of-function mutants and ectopic expression did not alter neuroepithelial cell number or proliferation. Notably, while ectopic expression of increases optic lobe volume and neuroblast numbers, our negative results suggest that these effects manifest earlier in development without persistent alteration of the neuroepithelium, through mechanisms that might be independent of neuroepithelial proliferation.
我们研究了氧感应非典型鸟苷酸环化酶亚基Gyc89Db在发育中的大脑中的作用。尽管它在果蝇幼虫视叶的缺氧神经上皮中表达，但功能丧失突变体和异位表达并未改变神经上皮细胞的数量或增殖。值得注意的是，虽然Gyc89Db的异位表达增加了视叶体积和成神经细胞数量，但我们的阴性结果表明，这些影响在发育早期就已显现，且没有通过可能独立于神经上皮增殖的机制对神经上皮产生持续改变。
