Babaei Parvin, Dastras Adele, Tehrani Bahram Soltani, Pourali Roudbaneh Shiva
Cellular & Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Neuroscience Research Center, Guilan University of Medical Sciences, Rasht, Iran.
J Menopausal Med. 2017 Dec;23(3):182-189. doi: 10.6118/jmm.2017.23.3.182. Epub 2017 Dec 29.
Ovarian hormones have been shown to regulate body weight, intra-abdominal fat accumulation and plasma level of cytokines. The aim of this study was to investigate the effect of estrogen replacement therapy on visceral adipose tissue, plasma level of apelin, lipid profiles, and glucose in ovariectomized (OVX) rats.
Thirty female Wistar rats were divided into OVX (n = 20) and sham (n = 10) groups. OVX rats were subdivided into estrogen replacement therapy (OVX+est; n = 10) receiving 17 β-estradiol valerates (30 µg/kg, s.c., 5 day/week, for eight weeks), and vehicle control group receiving sesame oil same as experiment group (OVX+ses oil; n = 10). After the treatments, all groups were sacrificed and blood samples were collected, visceral fats were taken from the abdominal cavity and weighed immediately. Apelin were measured using enzyme-linked immunosorbent assay kits. Lipid profiles and glucose were measured using the enzymatic colorimetric method. Data were analyzed with one-way analysis of variance and ( < 0.05) determined as the statistical significance level.
After eight weeks, body weight, body mass index (BMI), visceral fat, apelin and lipid profiles ( < 0.01) were increased significantly in OVX rats compared to sham group. Treatment with estrogen leads to significant reduction in body weight and BMI ( < 0.05), there was no significant change in serum apelin level in OVX+est rats compared to OVX+ses.
These results suggest that estradiol replacement therapy successfully attenuated some of the metabolic syndrome components, and apelin does not probably stand as a mediator of these physiological functions.
卵巢激素已被证明可调节体重、腹部脂肪堆积和细胞因子的血浆水平。本研究的目的是探讨雌激素替代疗法对去卵巢（OVX）大鼠内脏脂肪组织、血浆apelin水平、血脂谱和血糖的影响。
将30只雌性Wistar大鼠分为OVX组（n = 20）和假手术组（n = 10）。OVX大鼠再分为接受戊酸雌二醇（30 μg/kg，皮下注射，每周5天，共8周）的雌激素替代治疗组（OVX+est；n = 10）和接受与实验组相同芝麻油的溶剂对照组（OVX+芝麻油；n = 10）。治疗后，处死所有组并采集血样，从腹腔取出内脏脂肪并立即称重。使用酶联免疫吸附测定试剂盒测量apelin。使用酶促比色法测量血脂谱和血糖。数据采用单因素方差分析进行分析，以P < 0.05确定为统计学显著性水平。
八周后，与假手术组相比，OVX大鼠的体重、体重指数（BMI）、内脏脂肪、apelin和血脂谱（P < 0.01）显著增加。雌激素治疗导致体重和BMI显著降低（P < 0.05），与OVX+芝麻油组相比，OVX+est组大鼠血清apelin水平无显著变化。
这些结果表明，雌二醇替代疗法成功减轻了一些代谢综合征成分，apelin可能不是这些生理功能的介质。
