J&J Johnson & Johnson Consumer Inc., Skillman, NJ, USA.
Laboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, Faculty of Biology, Medicine and Health, School of Health Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Exp Dermatol. 2018 Aug;27(8):833-836. doi: 10.1111/exd.13499. Epub 2018 Feb 19.
Acne skin demonstrates increased transepidermal water loss (TEWL) compared with healthy skin, which may be due, in part, to altered ceramide (CER) levels. We analysed ceramides in the stratum corneum of healthy and acne skin, and studied seasonal variation over the course of a year. Using ultraperformance liquid chromatography with electrospray ionisation and tandem mass spectrometry (UPLC/ESI-MS/MS), we identified 283 ceramides. Acne-affected skin demonstrated overall lower levels of ceramides, with notable reductions in CER[NH] and CER[AH] ceramides, as well as the acylceramides CER[EOS] and CER[EOH]; these differences were more apparent in the winter months. Lower ceramide levels reflected an increase in TEWL in acne, compared with healthy skin, which partly resolves in the summer. Individual ceramide species with 18-carbon 6-hydroxysphingosine (H) bases (including CER[N(24)H(18)], CER[N(26)H(18)], CER[A(24)H(18)], CER[A(26)H(18)]) were significantly reduced in acne skin, suggesting that CER[NH] and CER[AH] species may be particularly important in a healthy skin barrier.
痤疮皮肤的经表皮水分流失（TEWL）高于健康皮肤，这可能部分归因于神经酰胺（CER）水平的改变。我们分析了健康和痤疮皮肤的角质层中的神经酰胺，并研究了一年中季节变化的情况。我们使用超高效液相色谱-电喷雾离子化-串联质谱法（UPLC/ESI-MS/MS）鉴定了 283 种神经酰胺。痤疮皮肤的神经酰胺总体水平较低，CER[NH]和 CER[AH]神经酰胺以及酰基神经酰胺 CER[EOS]和 CER[EOH]明显减少；这些差异在冬季更为明显。与健康皮肤相比，较低的神经酰胺水平反映了痤疮皮肤 TEWL 的增加，而在夏季则部分缓解。具有 18 个碳 6-羟基神经鞘氨醇（H）基的单个神经酰胺种类（包括 CER[N（24）H（18）]、CER[N（26）H（18）]、CER[A（24）H（18）]、CER[A（26）H（18）]）在痤疮皮肤中显著减少，这表明 CER[NH]和 CER[AH]种类在健康皮肤屏障中可能特别重要。
