Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, WA, 99350, USA.
Department of Plant Pathology, North Dakota State University, 306 Walster Hall, Fargo, ND, 58102, USA.
Arch Virol. 2018 Apr;163(4):1019-1030. doi: 10.1007/s00705-018-3714-0. Epub 2018 Jan 22.
The fungus, Erysiphe necator Schw., is an important plant pathogen causing powdery mildew disease in grapevines worldwide. In this study, high-throughput sequencing of double-stranded RNA extracted from the fungal tissue combined with bioinformatics was used to examine mycovirus-related sequences associated with E. necator. The results showed the presence of eight mycovirus-related sequences. Five of these sequences representing three new mycoviruses showed alignment with sequences of viruses classified in the genus Alphapartitivirus in the family Partitiviridae. Another three sequences representing three new mycoviruses showed similarity to classifiable members of the genus Mitovirus in the family Narnaviridae. These mycovirus isolates were named Erysiphe necator partitivirus 1, 2, and 3 (EnPV 1-3) and Erysiphe necator mitovirus 1, 2, and 3 (EnMV 1-3) reflecting their E. necator origin and their phylogenetic affiliation with other mycoviruses.
真菌，粉孢革菌（Erysiphe necator Schw.），是一种引起葡萄藤白粉病的重要植物病原体。在这项研究中，使用从真菌组织中提取的双链 RNA 的高通量测序结合生物信息学方法，研究了与 E. necator 相关的真菌病毒相关序列。结果表明存在 8 种与真菌病毒相关的序列。其中 5 个序列代表 3 种新的真菌病毒，与分类在二分病毒科（Partitiviridae）的 Alphapartitivirus 属中的病毒序列具有相似性。另外 3 个序列代表 3 种新的真菌病毒，与 Narnaviridae 科的可分类成员 Mitovirus 属具有相似性。这些真菌病毒分离物被命名为粉孢革菌二分病毒 1、2 和 3（EnPV 1-3）和粉孢革菌微病毒 1、2 和 3（EnMV 1-3），反映了它们的 E. necator 起源以及与其他真菌病毒的系统发育关系。
