Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No.2 West Yuanmingyuan Rd., Haidian District, Beijing, 100193, People's Republic of China.
Virol J. 2019 Sep 11;16(1):113. doi: 10.1186/s12985-019-1213-x.
Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. In recent years, mycoviruses from fungi have attracted increasing attention due to their effects on their hosts, but Trichoderma mycoviruses have not been the subject of extensive study. We sought to discover novel mycoviruses from Trichoderma spp. and to determine the effects of the biocontrol function of Trichoderma spp.
Mycoviruses were screened by dsRNA extraction and metagenomic analysis. RT-PCR, 5' RACE, and 3' RACE were used to obtain the genome sequence. MEGA software was used to classify the new mycovirus. The effects of the identified mycovirus on the biological properties of the host strain 525 were evaluated using cucumber plants and Fusarium oxysporum f. sp. cucumerinum.
A novel mycovirus, Trichoderma harzianum mycovirus 1 (ThMV1) (accession number MH155602), was discovered in Trichoderma harzianum strain 525, a soil-borne fungus collected from Inner Mongolia, China. The mycovirus exhibited a double-stranded RNA (dsRNA) genome with a complete genome sequence of 3160 base pairs and two open reading frames (ORFs) on the negative strand. Phylogenetic analysis indicated that it belongs to an unclassified family of dsRNA mycoviruses. The removal of ThMV1 from the host 525 strain reduced host biomass production and improved the biocontrol capability of the host for Fusarium oxysporum f. sp. cucumerinum. At same time, the presence of ThMV1 improved the growth of cucumber.
ThMV1 is a new unclassified mycovirus found in T. harzianum. It not only affects the phenotype of the host strain but also reduces its biocontrol function, which sheds light on the interaction between the mycovirus and Trichoderma spp.
木霉属真菌被广泛应用于农业领域，作为生物防治剂来预防土传植物病害。近年来，真菌病毒因其对宿主的影响而引起了越来越多的关注，但木霉属真菌病毒尚未得到广泛研究。我们试图从木霉属真菌中发现新的真菌病毒，并确定木霉属真菌的生物防治功能的影响。
通过 dsRNA 提取和宏基因组分析筛选真菌病毒。使用 RT-PCR、5' RACE 和 3' RACE 获得基因组序列。使用 MEGA 软件对新的真菌病毒进行分类。通过黄瓜植株和尖孢镰刀菌（Fusarium oxysporum f. sp. cucumerinum）评估鉴定出的真菌病毒对宿主菌株 525 生物学特性的影响。
在中国内蒙古采集的一种土壤真菌木霉属 525 中发现了一种新的真菌病毒，即哈茨木霉真菌病毒 1（ThMV1）（登录号 MH155602）。该真菌病毒表现为双链 RNA（dsRNA）基因组，全长 3160 个碱基对，在负链上有两个开放阅读框（ORF）。系统发育分析表明，它属于未分类的 dsRNA 真菌病毒家族。从宿主 525 菌株中去除 ThMV1 降低了宿主生物量的产生，并提高了宿主对尖孢镰刀菌（Fusarium oxysporum f. sp. cucumerinum）的生物防治能力。同时，ThMV1 的存在促进了黄瓜的生长。
ThMV1 是在哈茨木霉中发现的一种新的未分类真菌病毒。它不仅影响宿主菌株的表型，还降低了其生物防治功能，这揭示了真菌病毒与木霉属真菌之间的相互作用。
