Engineering and Technology Center of Special Crop Breeding & utilization, Fujian Agricultural and Forestry University, Fujian, Province, P.R. China.
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, P.R. China.
Sci Rep. 2018 Jan 22;8(1):1321. doi: 10.1038/s41598-018-19517-5.
Wheat straw (WS) is a potential biomass for production of monomeric sugars. However, the enzymatic hydrolysis ratio of cellulose in WS is relatively low due to the presence of lignin and hemicellulose. To enhance the enzymatic conversion of WS, we tested the impact of three different pretreatments, e.g. sulfuric acid (HSO), sodium hydroxide (NaOH), and hot water pretreatments to the enzymatic digestions. Among the three pretreatments, the highest cellulose conversion rate was obtained with the 4% NaOH pretreatment at 121 °C (87.2%). In addition, NaOH pretreatment was mainly effective in removing lignin, whereas the HSO pretreatment efficiently removed hemicellulose. To investigate results of pretreated process for enhancement of enzyme-hydolysis to the WS, we used scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy to analyze structural changes of raw and treated materials. The structural analysis indicated that after HSO and NaOH pretreatments, most of the amorphous cellulose and partial crystalline cellulose were hydrolyzed during enzymatic hydrolysis. The findings of the present study indicate that WS could be ideal materials for production of monomeric sugars with proper pretreatments and effective enzymatic base hydrolysis.
小麦秸秆（WS）是生产单糖的潜在生物质。然而，由于木质素和半纤维素的存在，WS 中纤维素的酶水解率相对较低。为了提高 WS 的酶转化效率，我们测试了三种不同预处理方法（如硫酸（HSO）、氢氧化钠（NaOH）和热水预处理）对酶解的影响。在这三种预处理方法中，在 121°C 下用 4% NaOH 预处理得到的纤维素转化率最高（87.2%）。此外，NaOH 预处理主要有效去除木质素，而 HSO 预处理则有效地去除半纤维素。为了研究预处理过程对增强 WS 酶水解的效果，我们使用扫描电子显微镜、X 射线衍射和傅里叶变换红外光谱分析了原材料和处理材料的结构变化。结构分析表明，在 HSO 和 NaOH 预处理后，在酶解过程中，大部分无定形纤维素和部分结晶纤维素被水解。本研究结果表明，经过适当的预处理和有效的酶基础水解，WS 可以成为生产单糖的理想材料。
