用新的有序系数对基质中胶原蛋白组织进行量化。
Quantization of collagen organization in the stroma with a new order coefficient.
作者信息
Germann James A, Martinez-Enriquez Eduardo, Marcos Susana
机构信息
Visual Optics and Biophotonics Group, Instituto de Óptica "Daza de Valdés", Consejo Superior de Investigaciones Cientificas (IO, CSIC), C/Serrano, 121, Madrid 28006, Spain.
出版信息
Biomed Opt Express. 2017 Dec 8;9(1):173-189. doi: 10.1364/BOE.9.000173. eCollection 2018 Jan 1.
Abstract
Many optical and biomechanical properties of the cornea, specifically the transparency of the stroma and its stiffness, can be traced to the degree of order and direction of the constituent collagen fibers. To measure the degree of order inside the cornea, a new metric, the order coefficient, was introduced to quantify the organization of the collagen fibers from images of the stroma produced with a custom-developed second harmonic generation microscope. The order coefficient method gave a quantitative assessment of the differences in stromal collagen arrangement across the cornea depths and between untreated stroma and cross-linked stroma.
摘要
角膜的许多光学和生物力学特性，特别是基质的透明度及其硬度，都可追溯到组成胶原纤维的有序程度和方向。为了测量角膜内部的有序程度，引入了一种新的指标——有序系数，用于根据定制开发的二次谐波产生显微镜所生成的基质图像来量化胶原纤维的排列。有序系数法对整个角膜深度以及未处理基质和交联基质之间的基质胶原排列差异进行了定量评估。
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2
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3
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Invest Ophthalmol Vis Sci. 2016 Mar;57(3):992-1001. doi: 10.1167/iovs.15-18689.
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